MYO9B Antibody

Novus Biologicals | Catalog # NB100-41092

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Peptide ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all MYO9B Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-KNDFEPVKQSKAQ corresponding to internal region according to NP_004136.2, NP_001123537.1.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24466350).

Specificity

This antibody is expected to recognise both reported isoforms (NP_004136.2; NP_001123537.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for MYO9B Antibody

Western Blot: MYO9B Antibody [NB100-41092]

Western Blot: MYO9B Antibody [NB100-41092]

Western Blot: MYO9B Antibody [NB100-41092] - (2ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for MYO9B Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:2000

Western Blot

2 - 6 ug/ml
Application Notes
WB: Approx. 200 kDa band observed in human liver lysates (calculated MW of 243 kDa band according to NP_004136.2). Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 24466350).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MYO9B

Myosin IX belongs to the family of unconventional myosins, distinct from the classical myosins I and myosins II. Myosin IX, thought to be involved in signal transduction and leukocyte differentiation, includes several isoforms. The rat isoforms are myosin IX/Myr5 (230 kDa) and Myr7 (300 kDa), and the human isoforms are myosin IXa (MYO9A) and myosin IXb (MYO9B). The isoforms of myosin IX have similar overall domain structure and are expressed in many tissues and cell types, although they have distinct tissue expression patterns. The subcellular localization of class IX myosins appears to be partly cytoplasmic, and partly associated with membranes and the actin cytoskeleton. The expression level of myosins IX may vary during development and differentiation.

Alternate Names

CELIAC4, myosin IXB, myosin-IXb, MYR5Unconventional myosin-9b, unconventional myosin IXb

Entrez Gene IDs

4650 (Human)

Gene Symbol

MYO9B

UniProt

NP_004136.2 (Human)

Additional MYO9B Products

Product Documents for MYO9B Antibody

Certificate of Analysis

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Product Specific Notices for MYO9B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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