MyoD Antibody (MYD712)

Novus Biologicals | Catalog # NBP2-32883

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Single Cell Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # MYD712
View all MyoD Primary Antibodies »

Product Specifications

Immunogen

Recombinant human MyoD1 protein

Localization

Nuclear. Only nuclear staining should be considered as evidence of skeletal muscle differentiation.

Marker

Rhabdomyosarcoma Marker

Specificity

Recognizes a phosphor-protein of 45kDa, identified as MyoD1. This MAb does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilm s tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing s sarcomas and alveolar soft part sarcomas.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MyoD Antibody (MYD712)

Immunohistochemistry-Paraffin: MyoD Antibody (MYD712) [NBP2-32883]

Immunohistochemistry-Paraffin: MyoD Antibody (MYD712) [NBP2-32883]

Immunohistochemistry-Paraffin: MyoD1 Antibody (MYD712) [NBP2-32883] - Formalin-fixed, paraffin-embedded human rhabdomyosarcoma stained with MyoD1 MAb (MYD712)

Applications for MyoD Antibody (MYD712)

Application
Recommended Usage

Flow Cytometry

0.5-1ug/million cells

Immunocytochemistry/ Immunofluorescence

0.5-1ug/ml

Immunohistochemistry-Paraffin

0.5-1.0ug/ml

Single Cell Western

100 ug/ml
Application Notes
Use in Western blot reported in reported in scientific literature (PMID: 30890574).

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS with 0.05% BSA.

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: MyoD

The Myogenic determination gene (MyoD) was first identified by the virtue of its ability to convert embryonic mouse fibroblast cells to muscle cells. It was subsequently shown that forced expression of MyoD (human homolog is myf 3) gene in a wide variety of normal and neoplastic cells could either convert the cells to muscle cells or activate a set of the otherwise transcriptionally inactive muscle-specific genes in these cells. The regulatory domain of the MyoD gene product lies within a 70 amino acid region and comprises a basic DNA binding motif and a helix-loop-helix (HLH) dimerization motif. Subsequent studies identified three other genes whose products shared sequence homology for the basic HLH domain of MyoD. These are; myf5, myogenin (human homolog is myf4) and myf6 (also known as MRF4 and herculin).

Long Name

Myoblast Determination Protein 1

Alternate Names

bHLHc1, MYF3, MYOD1, PUM

Gene Symbol

MYOD1

UniProt

P15172

Additional MyoD Products

Product Documents for MyoD Antibody (MYD712)

Certificate of Analysis

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Product Specific Notices for MyoD Antibody (MYD712)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MyoD Antibody (MYD712)

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Protocols

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