Myotubularin Antibody (1C10) - Azide and BSA Free

Novus Biologicals | Catalog # H00004534-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1C10

Format

Azide and BSA Free
View all Myotubularin Primary Antibodies »

Product Specifications

Immunogen

MTM1 (AAH30779, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MASASTSKYNSHSLENESIKRTSRDGVNRDLTEAVPRLPGETLITDKEVIYICPFNGPIKGRVYITNYRLYLRSLETDSSLILDVPLGVISRIEKMGGAT

Specificity

MTM1 - myotubularin 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Myotubularin Antibody (1C10) - Azide and BSA Free

Western Blot: Myotubularin Antibody (1C10) [H00004534-M01]

Western Blot: Myotubularin Antibody (1C10) [H00004534-M01]

Western Blot: Myotubularin Antibody (1C10) [H00004534-M01] - Analysis of MTM1 expression in transfected 293T cell line by MTM1 monoclonal antibody (M01), clone 1C10.Lane 1: MTM1 transfected lysate(69.9 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: Myotubularin Antibody (1C10) [H00004534-M01]

Immunohistochemistry-Paraffin: Myotubularin Antibody (1C10) [H00004534-M01]

Immunohistochemistry-Paraffin: Myotubularin Antibody (1C10) [H00004534-M01] - Analysis of monoclonal antibody to MTM1 on formalin-fixed paraffin-embedded human pancreas. Antibody concentration 3 ug/ml.
Western Blot: Myotubularin Antibody (1C10) [H00004534-M01]

Western Blot: Myotubularin Antibody (1C10) [H00004534-M01]

Western Blot: Myotubularin Antibody (1C10) [H00004534-M01] - Analysis of MTM1 over-expressed 293 cell line, cotransfected with MTM1 Validated Chimera RNAi ( Cat # H00004534-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MTM1 monoclonal antibody (M01), clone 1C10 (Cat # H00004534-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Sandwich ELISA: Myotubularin Antibody (1C10) [H00004534-M01] - Detection limit for recombinant GST tagged MTM1 is approximately 1ng/ml as a capture antibody.

Applications for Myotubularin Antibody (1C10) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Sandwich ELISA

1:100-1:2000

Western Blot

1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for IHC-P, RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Myotubularin

This gene encodes a dual-specificity phosphatase that acts on both phosphotyrosine and phosphoserine. It is required for muscle cell differentiation and mutations in this gene have been identified as being responsible for X-linked myotubular myopathy.

Alternate Names

CG2, CNM, EC 3.1.3.48, MTMX, myotubular myopathy 1, myotubularin, myotubularin 1, XLMTM

Entrez Gene IDs

4534 (Human)

Gene Symbol

MTM1

OMIM

300 (Human)

UniProt

AAH30779 (Human)

Additional Myotubularin Products

Product Documents for Myotubularin Antibody (1C10) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Myotubularin Antibody (1C10) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Myotubularin Antibody (1C10) - Azide and BSA Free

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Protocols

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FAQs

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