NAA10 Antibody - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-19461
![Immunohistochemistry-Paraffin: NAA10 Antibody [NBP2-19461]](https://resources.rndsystems.com/images/products/NAA10-Antibody-Immunohistochemistry-Paraffin-NBP2-19461-img0003.jpg "Immunohistochemistry-Paraffin: NAA10 Antibody [NBP2-19461]")
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Key Product Details
Species Reactivity
Validated:
Human, Mouse
Predicted:
Bovine (97%), Rabbit (92%), Rat (91%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
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Product Specifications
Immunogen
Full length human NAA10 Recombinant protein.
Reactivity Notes
Xenopus laevis (82%).
Localization
Cytoplasm; Nucleus
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for NAA10 Antibody - Azide and BSA Free
Immunohistochemistry-Paraffin: NAA10 Antibody [NBP2-19461]
Immunohistochemistry-Paraffin: NAA10 Antibody [NBP2-19461] - Immunohistochemical analysis of paraffin-embedded PC13 xenograft, using antibody at 1:500 dilution.![Western Blot: NAA10 Antibody [NBP2-19461]](https://resources.rndsystems.com/images/products/NAA10-Antibody-Western-Blot-NBP2-19461-img0001.jpg "Western Blot: NAA10 Antibody [NBP2-19461]")
Western Blot: NAA10 Antibody [NBP2-19461]
Western Blot: NAA10 Antibody [NBP2-19461] - Sample (30 ug of whole cell lysate) A: H1299 transfected scramble siRNA B: H1299 transfected ARDIA siRNA 12% SDS PAGE gel, diluted at 1:5000.![Immunoprecipitation: NAA10 Antibody [NBP2-19461]](https://resources.rndsystems.com/images/products/NAA10-Antibody-Immunoprecipitation-NBP2-19461-img0008.jpg "Immunoprecipitation: NAA10 Antibody [NBP2-19461]")
Immunoprecipitation: NAA10 Antibody [NBP2-19461]
Immunoprecipitation: NAA10 Antibody [NBP2-19461] - Immunoprecipitation of ARD1A protein from HeLa whole cell extracts using 5 ug of ARD1A antibody. Western blot analysis was performed using ARD1A antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Immunocytochemistry/ Immunofluorescence: NAA10 Antibody [NBP2-19461] -
ARD1A antibody detects NAA10 protein at Cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in -20C 100% MeOH for 5 min.
Green: NAA10 protein stained by ARD1A antibody (NBP2-19461) diluted at 1:500.
Blue: Hoechst 33343 staining.
Western Blot: NAA10 Antibody [NBP2-19461] -
Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with ARD1A antibody (NBP2-19461) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: NAA10 Antibody [NBP2-19461] -
ARD1A antibody detects ARD1A protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and blotted with ARD1A antibody (NBP2-19461) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for NAA10 Antibody - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Immunoprecipitation
1:100-1:500
Western Blot
1:500-1:10000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Format
Azide and BSA Free
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: NAA10
Alternate Names
ARD1, ARD1 homolog A, N-acetyltransferase (S. cerevisiae), ARD1 homolog, N-acetyltransferase (S. cerevisiae), ARD1AARD1 homolog, N-acetyltransferase, EC 2.3.1.-, EC 2.3.1.88, MGC71248, N(alpha)-acetyltransferase 10, NatA catalytic subunit, N-acetyltransferase, N-acetyltransferase ARD1, human homolog of, N-alpha-acetyltransferase 10, NatA catalytic subunit, N-terminal acetyltransferase complex ARD1 subunit homolog A
Gene Symbol
NAA10
Additional NAA10 Products
Product Documents for NAA10 Antibody - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for NAA10 Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.Citations for NAA10 Antibody - Azide and BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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