![Immunocytochemistry/ Immunofluorescence: Nanog Antibody (1E6C4) - BSA Free [NBP1-47427]](https://resources.rndsystems.com/images/products/Nanog-Antibody-1E6C4-Immunocytochemistry-Immunofluorescence-NBP1-47427-img0003.jpg "Immunocytochemistry/ Immunofluorescence: Nanog Antibody (1E6C4) - BSA Free [NBP1-47427]")
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Key Product Details
Species Reactivity
Human
Applications
Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 1E6C4
Format
BSA Free
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Product Specifications
Immunogen
Purified recombinant fragment of human Nanog (amino acids 20-166) expressed in E. coli. [UniProt# Q9H9S0]
Localization
Nucleus
Marker
Embryonic Stem Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Nanog Antibody (1E6C4) - BSA Free
Immunocytochemistry/ Immunofluorescence: Nanog Antibody (1E6C4) - BSA Free [NBP1-47427]
Immunocytochemistry/Immunofluorescence: Nanog Antibody (1E6C4) [NBP1-47427] - Confocal immunofluorescence analysis of NTERA-2 cells (left) and HeLa cells (right) using anti-Nanog Mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin.![Western Blot: Nanog Antibody (1E6C4)BSA Free [NBP1-47427]](https://resources.rndsystems.com/images/products/Nanog-Antibody-1E6C4-Western-Blot-NBP1-47427-img0004.jpg "Western Blot: Nanog Antibody (1E6C4)BSA Free [NBP1-47427]")
Western Blot: Nanog Antibody (1E6C4)BSA Free [NBP1-47427]
Western Blot: Nanog Antibody (1E6C4) [NBP1-47427] - Western blot analysis using anti-Nanog monoclonal antibody against NTERA-2 cell lysate (lane 2).![Western Blot: Nanog Antibody (1E6C4)BSA Free [NBP1-47427]](https://resources.rndsystems.com/images/products/Nanog-Antibody-1E6C4-Western-Blot-NBP1-47427-img0005.jpg "Western Blot: Nanog Antibody (1E6C4)BSA Free [NBP1-47427]")
Western Blot: Nanog Antibody (1E6C4)BSA Free [NBP1-47427]
Western Blot: Nanog Antibody (1E6C4) [NBP1-47427] - Whole cell protein from NTERA-2 was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 mg/ml anti-Nanog in 1% milk. Protein was detected with an anti-mouse HRP secondary antibody using chemiluminescence.Applications for Nanog Antibody (1E6C4) - BSA Free
Application
Recommended Usage
ELISA
1:10000
Immunocytochemistry/ Immunofluorescence
1:200-1:1000
Western Blot
1:500-1:2000
Application Notes
This Nanog (1E6C4) antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence and ELISA.
Reviewed Applications
Read 1 review rated 3 using NBP1-47427 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Nanog
Long Name
Nanog Homeobox
Alternate Names
FLJ12581, FLJ40451, hNanog, homeobox protein NANOG, Homeobox transcription factor Nanog, homeobox transcription factor Nanog-delta 48, Nanog homeobox
Entrez Gene IDs
79923 (Human)
Gene Symbol
NANOG
UniProt
Additional Nanog Products
Product Documents for Nanog Antibody (1E6C4) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Nanog Antibody (1E6C4) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Customer Reviews for Nanog Antibody (1E6C4) - BSA Free (1)
3 out of 5
1 Customer Rating
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Application: Western BlotSample Tested: Human cancer cell whole cell lysateSpecies: HumanVerified Customer | Posted 08/22/2015
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
