Nanog Antibody (5A10) - BSA Free

Immunohistochemistry-Paraffin: Nanog Antibody (5A10) - BSA Free [NBP1-04320]

Immunohistochemistry-Paraffin: Nanog Antibody (5A10) [NBP1-04320] - Human seminoma tissue were incubated with anti-human Nanog (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodiumcitrate buffer and detected using Diaminobenzidine (DAB)

Immunohistochemistry: Nanog Antibody (5A10) - BSA Free [NBP1-04320]

Nanog-Antibody-5A10-Immunohistochemistry-NBP1-04320-img0005.jpg

Immunocytochemistry/ Immunofluorescence: Nanog Antibody (5A10) - BSA Free [NBP1-04320] -

Immunocytochemistry/ Immunofluorescence: Nanog Antibody (5A10) - BSA Free [NBP1-04320] - Representative IF IHC-stained sections of MDBMSCC demonstrating the expression of pSTAT3 [(A), red] & EMA [(A), green] by cells within the tumor nests. There was a CSC subpopulation remonstrating nuclear co-expression of STAT3 [(B), red] & CD34 [(B), green], appearing as orange, on the endothelium of the microvessels within the stroma; & another subpopulation staining only positively for pSTAT3 within the stroma [(B), red]. Nuclear expression of NANOG [(C), red] was demonstrated on the endothelium of the microvessels which expressed CD34 [(C), green] within stroma. The NANOG+ cells [(C), red] that do not express CD34 were seen within the tumor nests & the stroma. SOX2 [(D), red] was also expressed by cells within tumor nests & the stroma, & the endothelium of the microvessels expressing CD34 [(D), green]. Nuclear expression of both SOX2 [(E), red] & SALL4 [(E), green], appearing as orange, was seen on the cells within the tumor nests & the stroma. Expression of both SOX2 [(F), red] & OCT4 [(F), green], appearing as orange, was seen on cells within the tumor nests & the stroma, & the endothelium of the microvessels within the stroma. pSTAT3 [(G), red] & membranous staining CD44 [(G), green] were co-expressed by cells within the tumor nests. Scale bars: 20 μm. Image collected & cropped by CiteAb from the following publication (http://journal.frontiersin.org/Article/10.3389/fsurg.2016.00046/abstract), licensed under a CC-BY license. Not internally tested by Novus Biologicals.