Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Nanog Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide which maps to a region between residues 250 and the C-terminus (residue 305) of mouse Nanog homeobox using the numbering given in TrEMBL entry Q80Z64 (GeneID 71950).

Reactivity Notes

Human reactivity reported in (PMID: 28228260), Customer review.

Marker

Embryonic Stem Cell Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Nanog Antibody - BSA Free

Western Blot: Nanog Antibody [NB100-588]

Western Blot: Nanog Antibody [NB100-588]

Nanog-Antibody-Western-Blot-NB100-588-img0010.jpg
Flow Cytometry: Nanog Antibody [NB100-588]

Flow Cytometry: Nanog Antibody [NB100-588]

Flow Cytometry: Nanog Antibody [NB100-588] - FACS analysis of Nanog expression in primary mouse hepatic stellate cell cultured for 7 days. Image from verified customer review.
Western Blot: Nanog Antibody [NB100-588]

Western Blot: Nanog Antibody [NB100-588]

Western Blot: Nanog Antibody [NB100-588] - Detection of Mouse Nanog by Western Blot and Immunoprecipitation.
Western Blot: Nanog Antibody [NB100-588]

Western Blot: Nanog Antibody [NB100-588]

Western Blot: Nanog Antibody [NB100-588] - The expression of pluripotency factor Nanog in human head and neck cancer cell lines cell lines FaDu and HN5. WB from a verified customer review.
Western Blot: Nanog Antibody [NB100-588]

Western Blot: Nanog Antibody [NB100-588]

Western Blot: Nanog Antibody [NB100-588] - Detection of mouse Nanog by western blot. Samples: Whole cell lysate (50 ug) from F9 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Nanog antibody NB100-588 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Western Blot: Nanog Antibody [NB100-588]

Western Blot: Nanog Antibody [NB100-588]

Nanog-Antibody-Western-Blot-NB100-588-img0009.jpg
Flow Cytometry: Nanog Antibody [NB100-588]

Flow Cytometry: Nanog Antibody [NB100-588]

Flow Cytometry: Nanog Antibody [NB100-588] - staining of NTERA-2 cells using NB100-588 at a 1:50 dilution detected using Dylight-488 conjugated goat anti-rabbit secondary antibody.
Nanog Antibody

Western Blot: Nanog Antibody [NB100-588] -

Western Blot: Nanog Antibody [NB100-588] - HIFs are required for hypoxia-induced expression of pluripotency factorsA-C. Breast cancer cell lines were exposed to 20% or 1% O2 for 24 h & NANOG (A), KLF4 (B), & SOX2 (C) mRNA levels were determined by RT-qPCR, relative to 18S rRNA, & normalized to the mean value for MDA-MB-231 cells (MDA231) at 20% O2 (mean ± SEM; n = 3). *P < 0.05, **P < 0.01, ***P < 0.001 vs. same cell line at 20% O2 by Student's t test. D & E. HCC-1954 (D) & MCF-7 (E) subclones, which were stably transfected with an expression vector encoding a non-targeting control (NTC) shRNA, or vector encoding shRNA targeting HIF-1 alpha (sh1 alpha ) or HIF-2 alpha (sh2 alpha ), or vectors encoding shRNAs targeting both HIF-1 alpha & HIF-2 alpha (DKD), were exposed to 20% or 1% O2 for 24 h & RT-qPCR was performed to determine NANOG (D) or KLF4 (E) mRNA levels relative to 18S rRNA. The results were normalized to NTC at 20% O2 (mean ± SEM; n = 3). *P < 0.05, **P < 0.01, ***P < 0.001 vs. NTC at 20% O2; #P < 0.05, ##P < 0.01, ###P < 0.001 vs. NTC at 1% O2 by ANOVA. F. ZR75.1 cells treated with vehicle or digoxin (200 nM) were exposed to 20% or 1% O2 for 24 h & SOX2 mRNA was measured (mean ± SEM; n = 3). *P < 0.05, **P < 0.01 vs. NTC at 20% O2; ###P < 0.001 vs. NTC at 1% O2 by ANOVA. G & H. NTC & DKD subclones of HCC-1954 (G) & MCF-7 (H) were exposed to 20% or 1% O2 for 48 h, whole cell lysates were prepared, & immunoblot assays were performed to analyze HIF-1 alpha, HIF-2 alpha, NANOG & KLF4 protein expression. Actin was also analyzed as a loading control. I. ZR75.1 cells were treated with vehicle or digoxin (200 nM), exposed to 20% or 1% O2 for 48 h, & HIF-1 alpha, NANOG & SOX2 immunoblot assays were performed. Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.11743), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Nanog Antibody - BSA Free

Application
Recommended Usage

Flow Cytometry

1:150

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
WB from a verified customer review, PMID ( 28228260). ICC/IF reported in (PMID: 22505032). Nanog antibody validated for FLOW from a verified customer review.

Reviewed Applications

Read 4 reviews rated 3.8 using NB100-588 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Nanog

Nanog is one of the molecular markers suitable for recognizing the undifferentiated state of stem cells in the mouse and human. It is a divergent homeodomain protein that directs propagation of undifferentiated ES cells. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Nanog-deficient ES cells lose pluripotency and differentiate into extraembryonic endoderm lineage.

Long Name

Nanog Homeobox

Alternate Names

FLJ12581, FLJ40451, hNanog, homeobox protein NANOG, Homeobox transcription factor Nanog, homeobox transcription factor Nanog-delta 48, Nanog homeobox

Entrez Gene IDs

71950 (Mouse)

Gene Symbol

NANOG

UniProt

Q80Z64

Additional Nanog Products

Product Documents for Nanog Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Nanog Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Nanog Antibody - BSA Free

Customer Reviews for Nanog Antibody - BSA Free (4)

3.8 out of 5
4 Customer Ratings
5 Stars
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4 Stars
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3 Stars
25%
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1 Stars
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Customer Images

Showing  1 - 4 of 4 reviews Showing All
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  • Nanog Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Human head and neck cancer cell lines
    Species: Human
    Verified Customer | Posted 10/11/2018
    The expression of pluripotency factors Nanog, Sox2, and Oct4 in human HNSCC cell lines FaDu and HN5.
    Nanog Antibody - BSA Free NB100-588
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Human cancer cell whole cell lysate
    Species: Human
    Verified Customer | Posted 08/22/2015
    Nanog expression in human breast cancer cell lines. NB100-588
    Nanog Antibody - BSA Free NB100-588
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested:
    Species: Mouse
    Verified Customer | Posted 04/29/2014
    FACS analysis of Nanog expression in primary mouse hepatic stellate cell cultured for 7 days.
    Nanog Antibody - BSA Free NB100-588
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: HUVEC, MCF7, JURKAT, Sample Amount: 2-30 ug
    Species: Human
    Verified Customer | Posted 06/08/2011
    Nanog Antibody - BSA Free NB100-588

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Protocols

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