NCF1 Antibody

Novus Biologicals | Catalog # NBP2-26113

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Canine (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all NCF1 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-DEAEDPDPNYAGEP corresponding to internal region according to NP_035006.3.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for NCF1 Antibody

Western Blot: NCF1 Antibody [NBP2-26113]

Western Blot: NCF1 Antibody [NBP2-26113]

Western Blot: NCF1 Antibody [NBP2-26113] - Staining of Daudi cell lysate with antibody at 1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: NCF1 Antibody [NBP2-26113]

Immunocytochemistry/ Immunofluorescence: NCF1 Antibody [NBP2-26113]

Immunocytochemistry/Immunofluorescence: NCF1 Antibody [NBP2-26113] - Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunocytochemistry/ Immunofluorescence: NCF1 Antibody [NBP2-26113]

Immunocytochemistry/ Immunofluorescence: NCF1 Antibody [NBP2-26113]

Immunocytochemistry/Immunofluorescence: NCF1 Antibody [NBP2-26113] - Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Applications for NCF1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Peptide ELISA

Detection limit 1:2000

Western Blot

1 - 3 ug/ml

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NCF1

NCF1, along with NCF2 and a membrane bound cytochrome b558, is required for activation of the latent NADPH oxidase necessary for superoxide production. Defects in NCF1 are the cause of autosomal cytochrome-b-positive chronic granulomatous disease type 1 (CGD). NCF1 is a cytosolic subunit of the multi-protein complex known as NADPH oxidase found in neutrophils.

Alternate Names

47 kDa neutrophil oxidase factor, FLJ79451, NADPH oxidase organizer 2, NCF-1, NCF1A, neutrophil cytosol factor 1,47 kDa autosomal chronic granulomatous disease protein, neutrophil cytosolic factor 1, neutrophil cytosolic factor 1 (47kD, chronic granulomatous disease, autosomal1), neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1), Neutrophil NADPH oxidase factor 1, Nox organizer 2, NOXO2NCF-47K, Nox-organizing protein 2, p47phox, SH3 and PX domain-containing protein 1A, SH3PXD1Ap47-phox

Entrez Gene IDs

653361 (Human); 17969 (Mouse); 114553 (Rat)

Gene Symbol

NCF1

Additional NCF1 Products

Product Documents for NCF1 Antibody

Certificate of Analysis

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Product Specific Notices for NCF1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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