NCOA3/AIB1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-77785

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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all NCOA3 Primary Antibodies »

Product Specifications

Immunogen

This affinity purified NCOA3/AIB1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human NCOA3/AIB1/AIB1 isoform a protein. (Uniprot: Q9Y6Q9)

Reactivity Notes

A BLAST analysis was used to suggest cross-reactivity with NCOA3/AIB1 from human and macaque sources based on a 100% homology with the immunizing sequence. Expect partial reactivity with NCOA3/AIB1 from dog, bovine, mouse and rat sources based on partial sequence homology. Cross-reactivity with NCOA3/AIB1 from other sources has not been determined.

Specificity

This antibody reacts with several isoforms of human NCOA3/AIB1 protein (a, b, d, e and f).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This product was affinity purified from monospecific antiserum by immunoaffinity chromatography

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for NCOA3/AIB1 Antibody - BSA Free

Western Blot: NCOA3/AIB1 Antibody [NBP1-77785]

Western Blot: NCOA3/AIB1 Antibody [NBP1-77785]

Western Blot: NCOA3/AIB1 Antibody [NBP1-77785] - Detection of AIB1 in mouse liver nuclear extract (lane 1), transient transfected 293 cell lysate (lane 2), HeLa whole cell lysate (lane 3) and mouse thyroid cell nuclear extract (lane 4). The band at ~148 kDa, indicated by the arrowhead, corresponds to AIB1. Mouse AIB1(lanes 1 and 4) appear as ~105 kDa bands. Pre-incubation of antibody with the immunizing peptide blocks detection of specific band staining (not shown). Personal communication, H. Ying, NCI, Bethesda, MD.
Immunohistochemistry: NCOA3/AIB1 Antibody [NBP1-77785]

Immunohistochemistry: NCOA3/AIB1 Antibody [NBP1-77785]

Immunohistochemistry: NCOA3/AIB1 Antibody [NBP1-77785] - Tissue: skin spleen. Fixation: formalin fixed paraffin embedded. Primary antibody: Anti-NCOA3 at 5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: NCOA-3 as precipitated red signal with hematoxylin purple nuclear counterstain.
Immunohistochemistry: NCOA3/AIB1 Antibody [NBP1-77785]

Immunohistochemistry: NCOA3/AIB1 Antibody [NBP1-77785]

Immunohistochemistry: NCOA3/AIB1 Antibody [NBP1-77785] - Tissue: skin spleen. Fixation: formalin fixed paraffin embedded. Primary antibody: Anti-NCOA3 at 5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: NCOA-3 as precipitated red signal with hematoxylin purple nuclear counterstain.
NCOA3/AIB1 Antibody

NCOA3/AIB1 Antibody

Immunohistochemistry of NCOA3/AIB1 Antibody. Tissue: skin spleen. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-NCOA3 at 5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: NCOA-3 as precipitated red signal with hematoxylin purple nuclear counterstain.
NCOA3/AIB1 Antibody

NCOA3/AIB1 Antibody

Immunohistochemistry of NCOA3/AIB1 Antibody. Tissue: skin spleen. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-NCOA3 at 5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: NCOA-3 as precipitated red signal with hematoxylin purple nuclear counterstain.

Applications for NCOA3/AIB1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:175000

Immunohistochemistry

5ug/ml

Immunoprecipitation

1:100

Western Blot

1:500-1:1000
Application Notes
This affinity purified antibody has been tested for use in ELISA, Immunohistochemistry, and western blotting. Specific conditions for reactivity should be optimized by the end user.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: NCOA3

AIB1 /SRC3 (nuclear receptor coactivator 3/steroid receptor coactivator 3 (SRC3)) is a 155 kD member of the p160/steroid receptor coactivator family containing a nuclear localization sequence, bHLH, and PAS domain. There are two reported isoforms of this protein. AIB1 /SRC3 is localized mainly in the cytoplasm, with weak nuclear expression. The protein is translocated to the nucleus upon phosphorylation. AIB1 /SRC3 is widely expressed with high expression in the heart, skeletal muscle, pancreas, and placenta and low expression in the brain. AIB1 /SRC3 is expressed at very low levels in the lung, liver, and kidney and overexpressed in breast and ovarian cancers. This protein has histone acetyltransferase activity and functions as a transcriptional coactivator for steroid and nuclear hormone receptors. Phosphorylation by I kappaB kinase enhances coactivator function. Acetylation by CREB-BP disrupts interaction with nuclear receptors. AIB1 /SRC3 forms a multisubunit coactivation complex with NCOA2 IKKA, IKKB, IKBKG and histone acetyltransferase protein CREB binding protein and has also been shown to interact with PCAF.

Long Name

Nuclear Receptor Co-activator 3

Alternate Names

ACTR, AIB1, KAT13B, pCIP, RAC3, SRC-1, SRC-3, TRAM1

Entrez Gene IDs

8202 (Human)

Gene Symbol

NCOA3

UniProt

Q9Y6Q9

Additional NCOA3 Products

Product Documents for NCOA3/AIB1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for NCOA3/AIB1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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