NCOA3/AIB1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-90256

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all NCOA3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PSKVSNQDSKSPLGFYCDQNPVESSMCQSNSRDHLSDKESKESSVEGAENQRGPLESKGHKKLLQLLTCSSDDRGHSSLTNSPLDSSCKESSVSVTSPSGVSSSTSGGVSSTSNMHGSLLQEKHRILHKLL

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (86%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NCOA3/AIB1 Antibody - BSA Free

NCOA3/AIB1 Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: NCOA3/AIB1 Antibody - BSA Free [NBP1-90256]

Immunocytochemistry/ Immunofluorescence: NCOA3/AIB1 Antibody - BSA Free [NBP1-90256]

Staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: NCOA3/AIB1 Antibody [NBP1-90256]

Immunohistochemistry-Paraffin: NCOA3/AIB1 Antibody [NBP1-90256]

Immunohistochemistry-Paraffin: NCOA3/AIB1 Antibody [NBP1-90256] - Staining of human kidney shows strong nuclear positivity in cells in glomeruli and cells in tubules.
Immunohistochemistry-Paraffin: NCOA3/AIB1 Antibody [NBP1-90256]

Immunohistochemistry-Paraffin: NCOA3/AIB1 Antibody [NBP1-90256]

Immunohistochemistry-Paraffin: NCOA3/AIB1 Antibody [NBP1-90256] - Staining of human lymph node shows strong nuclear positivity in germinal center and non-germinal center cells.
NCOA3/AIB1 Antibody - BSA Free Immunohistochemistry: NCOA3/AIB1 Antibody - BSA Free [NBP1-90256]

Immunohistochemistry: NCOA3/AIB1 Antibody - BSA Free [NBP1-90256]

Staining of human skeletal muscle shows moderate nuclear positivity in myocytes.
NCOA3/AIB1 Antibody - BSA Free Immunohistochemistry: NCOA3/AIB1 Antibody - BSA Free [NBP1-90256]

Immunohistochemistry: NCOA3/AIB1 Antibody - BSA Free [NBP1-90256]

Staining of human placenta shows strong nuclear positivity in trophoblastic cells.
NCOA3/AIB1 Antibody - BSA Free Western Blot: NCOA3/AIB1 Antibody - BSA Free [NBP1-90256]

Western Blot: NCOA3/AIB1 Antibody - BSA Free [NBP1-90256]

Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: Human cell line MCF-7
NCOA3/AIB1 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: NCOA3/AIB1 Antibody - BSA Free [NBP1-90256]

Chromatin Immunoprecipitation-exo-Seq: NCOA3/AIB1 Antibody - BSA Free [NBP1-90256]

ChIP-Exo-Seq composite graph for Anti-NCOA3 (NBP1-90256) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for NCOA3/AIB1 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NCOA3

NCOA3/SRC3 (nuclear receptor coactivator 3/steroid receptor coactivator 3 (SRC3)) is a 155 kD member of the p160/steroid receptor coactivator family containing a nuclear localization sequence, bHLH, and PAS domain. There are two reported isoforms of this protein. NCOA3/SRC3 is localized mainly in the cytoplasm, with weak nuclear expression. The protein is translocated to the nucleus upon phosphorylation. NCOA3/SRC3 is widely expressed with high expression in the heart, skeletal muscle, pancreas, and placenta and low expression in the brain. NCOA3/SRC3 is expressed at very low levels in the lung, liver, and kidney and overexpressed in breast and ovarian cancers. This protein has histone acetyltransferase activity and functions as a transcriptional coactivator for steroid and nuclear hormone receptors. Phosphorylation by I kappaB kinase enhances coactivator function. Acetylation by CREB-BP disrupts interaction with nuclear receptors. NCOA3/SRC3 forms a multisubunit coactivation complex with NCOA2 IKKA, IKKB, IKBKG and histone acetyltransferase protein CREB binding protein and has also been shown to interact with PCAF.

Long Name

Nuclear Receptor Co-activator 3

Alternate Names

ACTR, AIB1, KAT13B, pCIP, RAC3, SRC-1, SRC-3, TRAM1

Entrez Gene IDs

8202 (Human)

Gene Symbol

NCOA3

UniProt

Q9Y6Q9

Additional NCOA3 Products

Product Documents for NCOA3/AIB1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for NCOA3/AIB1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for NCOA3/AIB1 Antibody - BSA Free

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Protocols

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FAQs

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