NDC80 Antibody (9G3.23) - Azide and BSA Free

Novus Biologicals | Catalog # NB100-338

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Hamster, Marsupial

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Proximity Ligation Assay

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 9G3.23

Format

Azide and BSA Free
View all NDC80 Primary Antibodies »

Product Specifications

Immunogen

Human NDC80 protein consisting of amino acids 56-642.

Reactivity Notes

Kangaroo rat (100%).

Localization

Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NDC80 Antibody (9G3.23) - Azide and BSA Free

Western Blot: NDC80 Antibody (9G3.23) [NB100-338]

Western Blot: NDC80 Antibody (9G3.23) [NB100-338]

Western Blot: NDC80 Antibody (9G3.23) [NB100-338] - HEC1 antibody [9G3.23] detects HEC1 protein by western blot analysis. Various whole cell extracts and HeLa nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HEC1 antibody [9G3.23] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-193821) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.
Western Blot: NDC80 Antibody (9G3.23) [NB100-338]

Western Blot: NDC80 Antibody (9G3.23) [NB100-338]

Western Blot: NDC80 Antibody (9G3.23) [NB100-338] - WB analysis of NDC80 in U2OS Whole Cell Lysate. Verified customer review from 1DegreeBio.
Immunocytochemistry/ Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338]

Immunocytochemistry/ Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338]

Immunocytochemistry/Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - Mouse anti-Hec1 and rabbit anti-Hec1 (phospho Ser165) antibodies were used to co-stain MCF10A cells. Insets show kinetochores of misaligned chromosomes. DAPI staining shows chromatin.
Immunocytochemistry/ Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338]

Immunocytochemistry/ Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338]

Immunocytochemistry/Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - IF analysis of NDC80 in fixed human cell lines of RPE1, H4, and 42MGBA. Image courtesy of anonymous customer product review.
Immunocytochemistry/ Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338]

Immunocytochemistry/ Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338]

Immunocytochemistry/Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - IF analysis of NDC80 in PTK1 cells.Verified customer review from 1DegreeBio.
NDC80 Antibody (9G3.23)

Immunocytochemistry/ Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] -

Immunocytochemistry/ Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - NDC80 antibody [9G3.23] detects NDC80 protein at kinetochore by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NDC80 stained by NDC80 antibody [9G3.23] (NB100-338) diluted at 1:500.
Blue: Fluoroshield with DAPI.
NDC80 Antibody (9G3.23)

Immunocytochemistry/ Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] -

Immunocytochemistry/ Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - NDC80 antibody [9G3.23] detects NDC80 protein at kinetochore by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NDC80 stained by NDC80 antibody [9G3.23] (NB100-338) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000.
Blue: Fluoroshield with DAPI.
NDC80 Antibody (9G3.23)

Immunocytochemistry/ Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] -

Immunocytochemistry/ Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - NDC80 antibody [9G3.23] detects NDC80 protein at kinetochore by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NDC80 stained by NDC80 antibody [9G3.23] (NB100-338) diluted at 1:500.
Red: alpha Tubulin 4a, a cytoskeleton marker, stained by alpha Tubulin 4a antibody diluted at 1:500.
Blue: Hoechst 33342 staining.
NDC80 Antibody (9G3.23)

Immunocytochemistry/ Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] -

Immunocytochemistry/ Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - NDC80 antibody [9G3.23] detects NDC80 protein at kinetochore by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NDC80 stained by NDC80 antibody [9G3.23] (NB100-338) diluted at 1:500.
Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody diluted at 1:1000.
Blue: Fluoroshield with DAPI.
NDC80 Antibody (9G3.23) - Azide and BSA Free

Western Blot: NDC80 Antibody (9G3.23) [NB100-338] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NDC80 antibody [9G3.23] (NB100-338) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Applications for NDC80 Antibody (9G3.23) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Frozen

1:10 - 1:500

Immunoprecipitation

1-5 ug/ml

Western Blot

1:500-1:3000

Reviewed Applications

Read 5 reviews rated 4.6 using NB100-338 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NDC80

Human HEC1 is a coiled-coil protein that has been demonstrated to have a role in M-phase progression. It is essential for proper chromosome segregation. Its depletion results in the impairment of chromosome congression and results in spindle checkpoint activation.

Alternate Names

HEC1KNTC2, HECRetinoblastoma-associated protein HEC, Highly expressed in cancer protein, highly expressed in cancer, rich in leucine heptad repeats, highly expressed in cancer, rich in leucine heptad repeats (yeast), HsHec1, hsNDC80, kinetochore associated 2, Kinetochore protein Hec1, kinetochore protein NDC80 homolog, Kinetochore-associated protein 2, NDC80 homolog, kinetochore complex component (S. cerevisiae), TID3

Entrez Gene IDs

10403 (Human)

Gene Symbol

NDC80

UniProt

O14777 (Human)

Additional NDC80 Products

Product Documents for NDC80 Antibody (9G3.23) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for NDC80 Antibody (9G3.23) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for NDC80 Antibody (9G3.23) - Azide and BSA Free

Customer Reviews for NDC80 Antibody (9G3.23) - Azide and BSA Free (5)

4.6 out of 5
5 Customer Ratings
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Customer Images

Showing  1 - 5 of 5 reviews Showing All
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  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: RPE1 cells
    Species: Human
    Verified Customer | Posted 04/21/2016
    RPE1 cell stained with Ndc80
    NDC80 Antibody (9G3.23) - Azide and BSA Free NB100-338
  • Name: Andrew Cosgrove
    Application: Western Blot
    Sample Tested: U2OS whole cell lysate
    Species: Human
    Verified Customer | Posted 11/06/2013
    NDC80 Antibody (9G3.23) - Azide and BSA Free NB100-338
  • Name: Andrew Cosgrove
    Application: Immunofluorescence
    Sample Tested: PTK1 cells
    Species: Other
    Verified Customer | Posted 11/06/2013
    NDC80 Antibody (9G3.23) - Azide and BSA Free NB100-338
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested:
    Species: Human
    Verified Customer | Posted 10/04/2013
  • Name: Duane Compton
    Application: Immunofluorescence
    Sample Tested: Fixed cell lines (RPE1, H4, 42MGBA)
    Species: Human
    Verified Customer | Posted 06/06/2012
    Metaphase Spindle (alpha tubulin = green, anti-centromere = red, Hec1 = purple
    NDC80 Antibody (9G3.23) - Azide and BSA Free NB100-338

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