NDUFB5 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-31684

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Monkey (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all NDUFB5 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human NDUFB5. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rhesus Monkey (100%) and Chimpanzee (100%).

Localization

Mitochondrion inner membrane, Single-pass membrane protein, Matrix side

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NDUFB5 Antibody - BSA Free

Western Blot: NDUFB5 Antibody [NBP1-31684]

Western Blot: NDUFB5 Antibody [NBP1-31684]

Western Blot: NDUFB5 Antibody [NBP1-31684] - A. 30 ug 293T whole cell lysate/extract 12 % SDS-PAGE gel, antibody dilution 1:1000.
Immunocytochemistry/ Immunofluorescence: NDUFB5 Antibody [NBP1-31684]

Immunocytochemistry/ Immunofluorescence: NDUFB5 Antibody [NBP1-31684]

Immunocytochemistry/Immunofluorescence: NDUFB5 Antibody [NBP1-31684] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: NDUFB5 Antibody [NBP1-31684]

Immunohistochemistry-Paraffin: NDUFB5 Antibody [NBP1-31684]

Immunohistochemistry-Paraffin: NDUFB5 Antibody [NBP1-31684] - NDUFB6 antibody detects NDUFB6 protein at cytoplasm in rat muscle by immunohistochemical analysis. Sample: Paraffin-embedded rat muscle. NDUFB6 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: NDUFB5 Antibody [NBP1-31684]

Western Blot: NDUFB5 Antibody [NBP1-31684]

Western Blot: NDUFB5 Antibody [NBP1-31684] - 50 ug mouse brain lysate/extract 12 % SDS-PAGE gel, antibody dilution 1:1000.
Immunohistochemistry-Paraffin: NDUFB5 Antibody [NBP1-31684]

Immunohistochemistry-Paraffin: NDUFB5 Antibody [NBP1-31684]

Immunohistochemistry-Paraffin: NDUFB5 Antibody [NBP1-31684] - Paraffin-embedded HBL435 xenograft, using antibody at 1:500 dilution.
Immunohistochemistry-Frozen (Negative): NDUFB5 Antibody [NBP1-31684]

Immunohistochemistry-Frozen (Negative): NDUFB5 Antibody [NBP1-31684]

Immunohistochemistry-Frozen (Negative): NDUFB5 Antibody [NBP1-31684] - NDUFB5 antibody detects NDUFB5 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. NDUFB5 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for NDUFB5 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NDUFB5

The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Alternate Names

5 (16kD, SGDH), CISGDH, CI-SGDH, Complex I-SGDH, DKFZp686N02262, FLJ30597, MGC111204, MGC12314, NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 5, 16kDa, NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial, SGDH

Gene Symbol

NDUFB5

Additional NDUFB5 Products

Product Documents for NDUFB5 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for NDUFB5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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