NECAB1 Antibody (CL0575) - BSA Free

Novus Biologicals | Catalog # NBP2-52945

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (98%), Rat (98%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL0575

Format

BSA Free
View all NECAB1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from Q8N987, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for NECAB1 Antibody (CL0575) - BSA Free

NECAB1 Antibody (CL0575)

Immunohistochemistry-Paraffin: NECAB1 Antibody (CL0575) [NBP2-52945] -

Immunohistochemistry-Paraffin: NECAB1 Antibody (CL0575) [NBP2-52945] -Analysis in human cerebral cortex and pancreas tissues using NBP2-52945 antibody. Corresponding NECAB1 RNA-seq data are presented for the same tissues.
Western Blot: NECAB1 Antibody (CL0575) [NBP2-52945]

Western Blot: NECAB1 Antibody (CL0575) [NBP2-52945]

Western Blot: NECAB1 Antibody (CL0575) [NBP2-52945] - Lane 1: Marker [kDa], Lane 2: Human cerebral cortex lysate
NECAB1 Antibody (CL0575)

Immunohistochemistry-Paraffin: NECAB1 Antibody (CL0575) [NBP2-52945] -

Immunohistochemistry-Paraffin: NECAB1 Antibody (CL0575) [NBP2-52945] -Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.
NECAB1 Antibody (CL0575)

Immunohistochemistry-Paraffin: NECAB1 Antibody (CL0575) [NBP2-52945] -

Immunohistochemistry-Paraffin: NECAB1 Antibody (CL0575) [NBP2-52945] -Staining of human cerebellum shows moderate cytoplasmic positivity in neurons.
NECAB1 Antibody (CL0575)

Immunohistochemistry-Paraffin: NECAB1 Antibody (CL0575) [NBP2-52945] -

Immunohistochemistry-Paraffin: NECAB1 Antibody (CL0575) [NBP2-52945] -Staining of human kidney shows strong cytoplasmic positivity in cells in glomeruli.
NECAB1 Antibody (CL0575)

Immunohistochemistry-Paraffin: NECAB1 Antibody (CL0575) [NBP2-52945] -

Immunohistochemistry-Paraffin: NECAB1 Antibody (CL0575) [NBP2-52945] -Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

Applications for NECAB1 Antibody (CL0575) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NECAB1

The NECAB1 gene codes for a N-terminal EF-hand calcium-binding protein 1 that in isoform 1 is 351 amino acids long and 40 kDA and in isoform 2 is 100 amino acids long at 11 kDA. NECAB1 has been linked to diseases such as seizures, schizophrenia, alzheimer's disease, motor neuron disease, neuronitis, and neurodegenerative disease.

Alternate Names

EF hand calcium binding protein 1, EFCBP1, EF-hand calcium binding protein 1, EF-hand calcium-binding protein 1, neuronal calcium binding protein, Neuronal calcium-binding protein 1, N-terminal EF-hand calcium binding protein 1, N-terminal EF-hand calcium-binding protein 1, STIP-1, synaptotagmin interacting protein 1

Gene Symbol

NECAB1

Additional NECAB1 Products

Product Documents for NECAB1 Antibody (CL0575) - BSA Free

Certificate of Analysis

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Product Specific Notices for NECAB1 Antibody (CL0575) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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