Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # OTI5D1
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Product Specifications
Immunogen
Full length human recombinant protein of human NDN(NP_002478) produced in HEK293T cell.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Theoretical MW
35.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Necdin Antibody (OTI5D1)
![Western Blot: Necdin Antibody (OTI5D1) [NBP2-46117]](https://resources.rndsystems.com/images/products/Necdin-Antibody-OTI5D1-Western-Blot-NBP2-46117-img0018.jpg "Western Blot: Necdin Antibody (OTI5D1) [NBP2-46117]")
Western Blot: Necdin Antibody (OTI5D1) [NBP2-46117]
Western Blot: Necdin Antibody (OTI5D1) [NBP2-46117] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Necdin.![Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117]](https://resources.rndsystems.com/images/products/Necdin-Antibody-OTI5D1-Immunohistochemistry-NBP2-46117-img0017.jpg "Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117]")
Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117]
Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117]](https://resources.rndsystems.com/images/products/Necdin-Antibody-OTI5D1-Immunohistochemistry-NBP2-46117-img0002.jpg "Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117]")
Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117]
Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117]](https://resources.rndsystems.com/images/products/Necdin-Antibody-OTI5D1-Immunohistochemistry-NBP2-46117-img0003.jpg "Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117]")
Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117]
Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117]](https://resources.rndsystems.com/images/products/Necdin-Antibody-OTI5D1-Immunohistochemistry-NBP2-46117-img0004.jpg "Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117]")
Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117]
Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117]](https://resources.rndsystems.com/images/products/Necdin-Antibody-OTI5D1-Immunohistochemistry-NBP2-46117-img0005.jpg "Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117]")
Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117]
Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117] - Analysis of Carcinoma of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117]](https://resources.rndsystems.com/images/products/Necdin-Antibody-OTI5D1-Immunohistochemistry-NBP2-46117-img0006.jpg "Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117]")
Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117]
Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117]](https://resources.rndsystems.com/images/products/Necdin-Antibody-OTI5D1-Immunohistochemistry-NBP2-46117-img0007.jpg "Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117]")
Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117]
Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117]](https://resources.rndsystems.com/images/products/Necdin-Antibody-OTI5D1-Immunohistochemistry-NBP2-46117-img0008.jpg "Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117]")
Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117]
Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117]](https://resources.rndsystems.com/images/products/Necdin-Antibody-OTI5D1-Immunohistochemistry-NBP2-46117-img0009.jpg "Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117]")
Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117]
Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117]](https://resources.rndsystems.com/images/products/Necdin-Antibody-OTI5D1-Immunohistochemistry-NBP2-46117-img0010.jpg "Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117]")
Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117]
Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117]](https://resources.rndsystems.com/images/products/Necdin-Antibody-OTI5D1-Immunohistochemistry-NBP2-46117-img0011.jpg "Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117]")
Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117]
Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117]](https://resources.rndsystems.com/images/products/Necdin-Antibody-OTI5D1-Immunohistochemistry-NBP2-46117-img0012.jpg "Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117]")
Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117]
Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117]](https://resources.rndsystems.com/images/products/Necdin-Antibody-OTI5D1-Immunohistochemistry-NBP2-46117-img0013.jpg "Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117]")
Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117]
Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117]](https://resources.rndsystems.com/images/products/Necdin-Antibody-OTI5D1-Immunohistochemistry-NBP2-46117-img0014.jpg "Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117]")
Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117]
Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117]](https://resources.rndsystems.com/images/products/Necdin-Antibody-OTI5D1-Immunohistochemistry-NBP2-46117-img0015.jpg "Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117]")
Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117]
Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117]](https://resources.rndsystems.com/images/products/Necdin-Antibody-OTI5D1-Immunohistochemistry-NBP2-46117-img0016.jpg "Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117]")
Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117]
Immunohistochemistry: Necdin Antibody (OTI5D1) [NBP2-46117] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunofluorescence: Necdin Antibody (OTI5D1) [NBP2-46117]](https://resources.rndsystems.com/images/products/Necdin-Antibody-OTI5D1-Immunofluorescence-NBP2-46117-img0001.jpg "Immunofluorescence: Necdin Antibody (OTI5D1) [NBP2-46117]")
Immunofluorescence: Necdin Antibody (OTI5D1) [NBP2-46117]
Immunofluorescence: Necdin Antibody (OTI5D1) [NBP2-46117] - Analysis of HeLa cells.Applications for Necdin Antibody (OTI5D1)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:150
Western Blot
1:4000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Necdin
Alternate Names
HsT16328, necdin, necdin (mouse) homolog, necdin homolog (mouse), PWCR
Gene Symbol
NDN
Additional Necdin Products
Product Documents for Necdin Antibody (OTI5D1)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Necdin Antibody (OTI5D1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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