Nectin-2/CD112 Antibody (JG39-64)
Novus Biologicals | Catalog # NBP2-76852
Recombinant Monoclonal Antibody.
![Western Blot: Nectin-2/CD112 Antibody (JG39-64) [NBP2-76852]](https://beta-resources.rndsystems.com/images/products/Nectin-2-CD112-Antibody-JG39-64-Western-Blot-NBP2-76852-img0001.jpg "Western Blot: Nectin-2/CD112 Antibody (JG39-64) [NBP2-76852]")
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JG39-64 expressed in HEK293
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Product Specifications
Immunogen
Recombinant protein within Human Nectin-2/CD112 aa 370-538 / 538. (SwissProt: Q92692 Human; SwissProt: P32507 Mouse; SwissProt: Q5FVC5 Rat)
Localization
Cell membrane. Membrane.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit Nectin-2/CD112 Antibody (JG39-64) (NBP2-76852) is a recombinant monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Nectin-2/CD112 Antibody (JG39-64)
Western Blot: Nectin-2/CD112 Antibody (JG39-64) [NBP2-76852]
Western Blot: Nectin-2/CD112 Antibody (JG39-64) [NBP2-76852] - Analysis of Nectin 2 on K562 (1), MCF-7 (2) and SK-OV-3 (3) cells lysates using anti-Nectin 2 antibody at 1/500 dilution.![Immunocytochemistry/ Immunofluorescence: Nectin-2/CD112 Antibody (JG39-64) [NBP2-76852]](https://beta-resources.rndsystems.com/images/products/Nectin-2-CD112-Antibody-JG39-64-Immunocytochemistry-Immunofluorescence-NBP2-76852-img0003.jpg "Immunocytochemistry/ Immunofluorescence: Nectin-2/CD112 Antibody (JG39-64) [NBP2-76852]")
Immunocytochemistry/ Immunofluorescence: Nectin-2/CD112 Antibody (JG39-64) [NBP2-76852]
Immunocytochemistry/Immunofluorescence: Nectin-2/CD112 Antibody (JG39-64) [NBP2-76852] - ICC staining Nectin 2 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.![Immunohistochemistry: Nectin-2/CD112 Antibody (JG39-64) [NBP2-76852]](https://beta-resources.rndsystems.com/images/products/Nectin-2-CD112-Antibody-JG39-64-Immunohistochemistry-NBP2-76852-img0006.jpg "Immunohistochemistry: Nectin-2/CD112 Antibody (JG39-64) [NBP2-76852]")
Immunohistochemistry: Nectin-2/CD112 Antibody (JG39-64) [NBP2-76852]
Immunohistochemistry: Nectin-2/CD112 Antibody (JG39-64) [NBP2-76852] - Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Nectin 2 antibody. Counter stained with hematoxylin.![Flow Cytometry: Nectin-2/CD112 Antibody (JG39-64) [NBP2-76852]](https://beta-resources.rndsystems.com/images/products/Nectin-2-CD112-Antibody-JG39-64-Flow-Cytometry-NBP2-76852-img0007.jpg "Flow Cytometry: Nectin-2/CD112 Antibody (JG39-64) [NBP2-76852]")
Flow Cytometry: Nectin-2/CD112 Antibody (JG39-64) [NBP2-76852]
Flow Cytometry: Nectin-2/CD112 Antibody (JG39-64) [NBP2-76852] - Flow cytometric analysis of LOVO cells with Nectin 2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.![Immunocytochemistry/ Immunofluorescence: Nectin-2/CD112 Antibody (JG39-64) [NBP2-76852]](https://beta-resources.rndsystems.com/images/products/Nectin-2-CD112-Antibody-JG39-64-Immunocytochemistry-Immunofluorescence-NBP2-76852-img0002.jpg "Immunocytochemistry/ Immunofluorescence: Nectin-2/CD112 Antibody (JG39-64) [NBP2-76852]")
Immunocytochemistry/ Immunofluorescence: Nectin-2/CD112 Antibody (JG39-64) [NBP2-76852]
Immunocytochemistry/Immunofluorescence: Nectin-2/CD112 Antibody (JG39-64) [NBP2-76852] - ICC staining Nectin 2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.![Immunohistochemistry: Nectin-2/CD112 Antibody (JG39-64) [NBP2-76852]](https://beta-resources.rndsystems.com/images/products/Nectin-2-CD112-Antibody-JG39-64-Immunohistochemistry-NBP2-76852-img0004.jpg "Immunohistochemistry: Nectin-2/CD112 Antibody (JG39-64) [NBP2-76852]")
Immunohistochemistry: Nectin-2/CD112 Antibody (JG39-64) [NBP2-76852]
Immunohistochemistry: Nectin-2/CD112 Antibody (JG39-64) [NBP2-76852] - Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Nectin 2 antibody. Counter stained with hematoxylin.![Immunohistochemistry: Nectin-2/CD112 Antibody (JG39-64) [NBP2-76852]](https://beta-resources.rndsystems.com/images/products/Nectin-2-CD112-Antibody-JG39-64-Immunohistochemistry-NBP2-76852-img0005.jpg "Immunohistochemistry: Nectin-2/CD112 Antibody (JG39-64) [NBP2-76852]")
Immunohistochemistry: Nectin-2/CD112 Antibody (JG39-64) [NBP2-76852]
Immunohistochemistry: Nectin-2/CD112 Antibody (JG39-64) [NBP2-76852] - Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Nectin 2 antibody. Counter stained with hematoxylin.Applications for Nectin-2/CD112 Antibody (JG39-64)
Application
Recommended Usage
Flow Cytometry
1:50-1:100
Immunocytochemistry/ Immunofluorescence
1:50
Immunohistochemistry-Paraffin
1:50-1:200
Western Blot
1:500-1:2000
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Nectin-2/CD112
Long Name
Poliovirus Receptor Related 2
Alternate Names
CD112, HVEB, MPH, Nectin2, PRR2, PVRL2
Gene Symbol
NECTIN2
Additional Nectin-2/CD112 Products
Product Documents for Nectin-2/CD112 Antibody (JG39-64)
Product Specific Notices for Nectin-2/CD112 Antibody (JG39-64)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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