NEDD8 Antibody (5B8) - BSA Free

Novus Biologicals | Catalog # NBP2-37523

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5B8

Format

BSA Free
View all NEDD8 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human NEDD8 expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse NEDD8 Antibody (5B8) - BSA Free (NBP2-37523) is a monoclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for NEDD8 Antibody (5B8) - BSA Free

Western Blot: NEDD8 Antibody (5B8)BSA Free [NBP2-37523]

Western Blot: NEDD8 Antibody (5B8)BSA Free [NBP2-37523]

Western Blot: NEDD8 Antibody (5B8) [NBP2-37523] - Western blot analysis using NEDD8 mouse mAb against C6 (1) and Hela (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: NEDD8 Antibody (5B8) - BSA Free [NBP2-37523]

Immunocytochemistry/ Immunofluorescence: NEDD8 Antibody (5B8) - BSA Free [NBP2-37523]

Immunocytochemistry/Immunofluorescence: NEDD8 Antibody (5B8) [NBP2-37523] - Immunofluorescence analysis of Hela cells using NEDD8 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry: NEDD8 Antibody (5B8) - BSA Free [NBP2-37523]

Immunohistochemistry: NEDD8 Antibody (5B8) - BSA Free [NBP2-37523]

Immunohistochemistry: NEDD8 Antibody (5B8) [NBP2-37523] - Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using NEDD8 mouse mAb with DAB staining.
Flow Cytometry: NEDD8 Antibody (5B8) - BSA Free [NBP2-37523]

Flow Cytometry: NEDD8 Antibody (5B8) - BSA Free [NBP2-37523]

Flow Cytometry: NEDD8 Antibody (5B8) [NBP2-37523] - Flow cytometric analysis of Hela cells using NEDD8 mouse mAb (green) and negative control (red).
Western Blot: NEDD8 Antibody (5B8)BSA Free [NBP2-37523]

Western Blot: NEDD8 Antibody (5B8)BSA Free [NBP2-37523]

Western Blot: NEDD8 Antibody (5B8) [NBP2-37523] - Western blot analysis using NEDD8 mAb against human NEDD8 (AA: 1-81) recombinant protein. (Expected MW is 40 kDa)
ELISA: NEDD8 Antibody (5B8) - BSA Free [NBP2-37523]

ELISA: NEDD8 Antibody (5B8) - BSA Free [NBP2-37523]

ELISA: NEDD8 Antibody (5B8) [NBP2-37523] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for NEDD8 Antibody (5B8) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NEDD8

NEDD8 (Neural precursor cell-Expressed Developmentally Down-regulated genes) is an ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis (1). NEDD8 has been shown to conjugate to nuclear proteins in a manner analogous to ubiquination and sentrinization. The conjugation process is though to be catalyzed by thee enzymes; E1 (NEDD8-activation), E2 (NEDD8-conjugation) and E3 (NEDD8-ligation). NEDD8 is known to conjugate to Cul-4A and other cullins proteins via the carboxy-terminal Gly residue for ubiquitinylation (3) while NUB1 will negatively regulate its expression (4).

Long Name

Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 8

Alternate Names

Neddylin, Rub1

Gene Symbol

NEDD8

Additional NEDD8 Products

Product Documents for NEDD8 Antibody (5B8) - BSA Free

Certificate of Analysis

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Product Specific Notices for NEDD8 Antibody (5B8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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