Nephrin Antibody (Y17-R)

Novus Biologicals | Catalog # NBP1-30130

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # Y17-R
View all Nephrin Primary Antibodies »

Product Specifications

Immunogen

Peptide derived from the center of the fifth Ig-like domain of human Nephrin. Antibody recognizes the epitope located between Cys465 - Cys528.

Epitope

Cys465 - Cys528

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is immunoaffinity purified with immunogenic peptide as a ligand.

Scientific Data Images for Nephrin Antibody (Y17-R)

Western Blot: Nephrin Antibody (Y17-R) [NBP1-30130]

Western Blot: Nephrin Antibody (Y17-R) [NBP1-30130]

Nephrin-Antibody-Y17-R-Western-Blot-NBP1-30130-img0003.jpg
Immunocytochemistry/ Immunofluorescence: Nephrin Antibody (Y17-R) [NBP1-30130]

Immunocytochemistry/ Immunofluorescence: Nephrin Antibody (Y17-R) [NBP1-30130]

Immunocytochemistry/Immunofluorescence: Nephrin Antibody (Y17-R) [NBP1-30130] - Nephrin expression in HEK293 cells, visualized with clonal rabbit antiNephrin monospecific antibody. Primary antibody dilution - 1:70
Western Blot: Nephrin Antibody (Y17-R) [NBP1-30130]

Western Blot: Nephrin Antibody (Y17-R) [NBP1-30130]

Western Blot: Nephrin Antibody (I) [NBP1-30130] - Analysis of mouse nephrin: lane 1 - 50ng; lane 2 - 100ng; lane 3 -200ng of recombinant mouse nephrin.
Nephrin Antibody (Y17-R)

Western Blot: Nephrin Antibody (Y17-R) [NBP1-30130] -

Western Blot: Nephrin Antibody (Y17-R) [NBP1-30130] - Stiffness-mediated differentiation phenotype induces upregulation of podocyte-specific functional proteins.(A) Representative images of Western blots for five proteins critical for physiological function of podocytes. Images of the complete blots are shown in Figure S3. (B) Quantification of kidney podocyte protein markers. Highest upregulation of WT-1 was found on the 2 kPa gel (2.2 ± 0.3). Nephrin, podocin, & CD2AP showed similar trends. Synaptopodin did not show this trend & there was no statistical difference between softer gels & control. T-statistics showed statistically significant changes of the ratios of five protein markers after normalization by control (two-tails, alpha  = 0.05). The average t-statistics were 4.5 for the 2 kPa gel & 4.0 for the 5 kPa gel, which were significantly different relative to the control. Those for the 0.6 kPa and 13 kPa gels were 0.8 & 1.5, & the differences were not significant. Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/srep43934), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Nephrin Antibody (Y17-R)

Application
Recommended Usage

ELISA

1:50000-1:100000

Immunoprecipitation

1:10-1:500

Western Blot

1:5000
Application Notes
Use in IHC-P reported in scientific literature (PMID:34516901). For western blots, incubate the membrane with antibody diluted in blocking buffer for 2 hours at room temperature. Western immunoblotting solutions: Wash buffer: 1x Tris Buffered Saline (TBS). ; 0.1% Triton X-100. Blocking buffer: 1xTBS; 0.1% Triton X-100; 5% nonfat dry milk. Use in Immunohistochemistry reported in scientific literature (PMID: 25129444)..

Reviewed Applications

Read 1 review rated 4 using NBP1-30130 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

20mM Tris/HCl (pH 8.0) and 10 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nephrin

Nephrin is a member of the immunoglobulin family of cell adhesion molecules that localizes to opposing sites of the secondary foot processes formed by podocytes, a specialized epithelial cell that ensures size- and charge-selective ultrafiltration. The human nephrin gene maps to chromosome 19q13.1 and encodes a 1,241 amino acid protein that contains a transmembrane domain, 8 Ig-like modules, and 1 fibronectin III-like module. Nephrin is expressed in embryonic and adult kidneys and localizes to glomerular podocytes and the glomerular slit diaphragm. Nephrin stimulates mitogen-activated protein kinases and is enhanced by podocin, which binds to the cytoplasmic tail of nephrin. A293 cells treated with Phorbol-12-myristate-13-acetate can upregulate nephrin, suggesting that protein kinase C is part of an intracellular signalling system, which regulates nephrin.

Alternate Names

CNF, NPHS1

Entrez Gene IDs

4868 (Human); 54631 (Mouse); 64563 (Rat)

Gene Symbol

NPHS1

UniProt

Q9QZS7, O60500, Q9R044 (Rat)

Additional Nephrin Products

Product Documents for Nephrin Antibody (Y17-R)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Nephrin Antibody (Y17-R)

This antibody is immunoaffinity purified with immunogenic peptide as a ligand.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Immunoglobulin Superfamily CAMs

Citations for Nephrin Antibody (Y17-R)

Customer Reviews for Nephrin Antibody (Y17-R) (1)

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  • Name: Evren Azeloglu
    Application: Western Blot
    Sample Tested: Immortalized human podocytes
    Species: Human
    Verified Customer | Posted 04/28/2014
    Western Blot, rabbit anti-nephrin (G17-H) 1:1000. Sample: Immortalized human podocytes cultured for 72 hours at 37C.
    Nephrin Antibody (Y17-R) NBP1-30130

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Protocols

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