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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot, ELISA, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This affinity purified NEU2 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion near aa 100-125 of Human NEU2. (Uniprot: Q9Y3R4)
Reactivity Notes
This antibody reacts with human NEU2. Based on sequence we expect this antibody to react with neuraminidase from other sources, although specific reactivity has not been confirmed. Cross-reactivity against Neu1 has not yet been established.
Specificity
This antibody reacts with human NEU2. Based on sequence we expect this antibody to react with neuraminidase from other sources, although specific reactivity has not been confirmed. Cross-reactivity against Neu1 has not yet been established.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for NEU2 Antibody - BSA Free
Western Blot: NEU2 Antibody [NBP2-21649]
Western Blot: NEU2 Antibody [NBP2-21649] - Affinity Purified anti-Neu2 antibody to detect Neu-2 present in a lysate expressing human Neu2. Molecular weight marker indicates a band of the expected MW. The reactive lower molecular weight band is believed to represent a truncated form of this protein. The blot was incubated with a 1:500 dilution of the antibody at room temperature for 1 h followed by detection using InfraRed 800 labeled Goat-a-Rabbit IgG (H+L) diluted 1:1,000. InfraRed 800 fluorescence image was captured using the Infrared Imaging System Other detection systems will yield similar results.Western Blot: NEU2 Antibody [NBP2-21649]
Western Blot: NEU2 Antibody [NBP2-21649] - Affinity Purified anti-Neu2 antibody to detect recombinant His tagged Neu-2. Molecular weight marker indicates a single band of the expected MW. The blot was incubated with a 1:500 dilution of the antibody at room temperature for 1 h followed by detection using InfraRed Dye 800 labeled Goat-a-Rabbit IgG [H+L] diluted 1:1000. Other detection systems will yield similar results.NEU2 Antibody
Western blot analysis using Immunochemical's Affinity Purified anti-Neu2 antibody to detect recombinant His tagged Neu-2 (1.0 ug loaded). Molecular weight marker (not shown) indicates a single band of the expected MW (43 kDa). The blot was incubated with a 1:500 dilution of the antibody at room temperature for 1 h followed by detection using IRDye800 labeled Goat-a-Rabbit IgG [H&L] () diluted 1:1,000. IRDye800 fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.Applications for NEU2 Antibody - BSA Free
Application
Recommended Usage
ELISA
1:10000-1:50000
Western Blot
1:500-1:2000
Application Notes
This product is has been tested by western blot and ELISA and suitable for immunocytochemistry and immunoprecipitation, transfected cell culture, and primary cell culture. A single band of the expected apparent molecular weight (43 kDa) was observed at a 1:500 dilution incubated for 1 h at room temperature. A second lower molecular weight band may represent a truncated form of this protein. Neuraminidase is not very abundant in most tissues and its detection using this antibody may require further optimization. Researchers should determine optimal titers for other applications.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Format
BSA Free
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: NEU2
Long Name
Sialidase-2
Alternate Names
Cytosolic sialidase, EC 3.2.1.18
Gene Symbol
NEU2
UniProt
Additional NEU2 Products
Product Documents for NEU2 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for NEU2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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