Neuroligin 4X/NLGN4X Antibody (S98-7) - BSA Free
Novus Biologicals | Catalog # NBP2-42198
![Western Blot: Neuroligin 4X/NLGN4X Antibody (S98-7) [NBP2-42198]](https://resources.rndsystems.com/images/products/Neuroligin-4X-NLGN4X-Antibody-S98-7-Western-Blot-NBP2-42198-img0005.jpg "Western Blot: Neuroligin 4X/NLGN4X Antibody (S98-7) [NBP2-42198]")
Key Product Details
Species Reactivity
Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # S98-7
Format
BSA Free
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Product Specifications
Immunogen
Fusion protein amino acids 782-945 (intracellular C-terminus) of mouse Neuroligin 4. ~45% identity with Neuroligin-1, -2 and -3.
Localization
Cell Membrane, Cell Junction
Specificity
Approx 45% identity with Neuroligin-1, -2 and -3.Detects approx 25-130kDa.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Neuroligin 4X/NLGN4X Antibody (S98-7) - BSA Free
Western Blot: Neuroligin 4X/NLGN4X Antibody (S98-7) [NBP2-42198]
Western Blot: Neuroligin 4X/NLGN4X Antibody (S98-7) [NBP2-42198] - Western Blot analysis of Rat Brain Membrane showing detection of ~125-130 kDa Neuroligin 4X/NLGN4X protein using Mouse Anti-Neuroligin 4X/NLGN4X Monoclonal Antibody, Clone S98-7 (NBP2-42198). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Neuroligin 4X/NLGN4X Monoclonal Antibody (NBP2-42198) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~125-130 kDa.![Immunocytochemistry/ Immunofluorescence: Neuroligin 4X/NLGN4X Antibody (S98-7) [NBP2-42198]](https://resources.rndsystems.com/images/products/Neuroligin-4X-NLGN4X-Antibody-S98-7-Immunocytochemistry-Immunofluorescence-NBP2-42198-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Neuroligin 4X/NLGN4X Antibody (S98-7) [NBP2-42198]")
Immunocytochemistry/ Immunofluorescence: Neuroligin 4X/NLGN4X Antibody (S98-7) [NBP2-42198]
Immunocytochemistry/Immunofluorescence: Neuroligin 4X/NLGN4X Antibody (S98-7) [NBP2-42198] - Tissue: Dentate Gyrus. Species: Mouse. Fixation: formaldehdye-fixed parrafin embedded. Primary Antibody: Mouse Anti-Neuroligin 4 Monoclonal Antibody at 1:100. Secondary Antibody: FITC Goat Anti-Mouse (green). Counterstain: DAPI (blue) nuclear stain at 1:1000. Courtesy of: Rachel Reith, NIH/NIMH.Applications for Neuroligin 4X/NLGN4X Antibody (S98-7) - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:100
Western Blot
1:1000
Application Notes
1 ug/ml of Neuroligin 4 Antibody was sufficient for detection of Neuroligin 4 in 20 ug of mouse brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4), 50% Glycerol
Format
BSA Free
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Neuroligin 4X/NLGN4X
Alternate Names
ASPGX2, AUTSX2, HNL4X, HNLX, NL4, NLGN4, NLGN4X
Gene Symbol
NLGN4X
UniProt
Additional Neuroligin 4X/NLGN4X Products
Product Documents for Neuroligin 4X/NLGN4X Antibody (S98-7) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Neuroligin 4X/NLGN4X Antibody (S98-7) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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