Neuronal Pentraxin 2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-76899

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Neuronal Pentraxin 2 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 16 amino acid synthetic peptide near the center of the human NPTX2. The immunogen is located within amino acids 170 - 220 of NPTX2.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Guinea pig (88%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Neuronal Pentraxin 2 Antibody - BSA Free

Western Blot: Neuronal Pentraxin 2 AntibodyBSA Free [NBP1-76899]

Western Blot: Neuronal Pentraxin 2 AntibodyBSA Free [NBP1-76899]

Western Blot: Neuronal Pentraxin 2 Antibody [NBP1-76899] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: Neuronal Pentraxin 2 NBP1-76899 (2 ug/mL), Neuronal Pentraxin 2 (4 ug/mL), and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot: Neuronal Pentraxin 2 AntibodyBSA Free [NBP1-76899]

Western Blot: Neuronal Pentraxin 2 AntibodyBSA Free [NBP1-76899]

Western Blot: Neuronal Pentraxin 2 Antibody [NBP1-76899] - Mouse brain tissue lysate with NPTX2 antibody at (A) 0.5 and (B) 1 ug/mL.
Western Blot: Neuronal Pentraxin 2 AntibodyBSA Free [NBP1-76899]

Western Blot: Neuronal Pentraxin 2 AntibodyBSA Free [NBP1-76899]

Western Blot: Neuronal Pentraxin 2 Antibody [NBP1-76899] - Mouse brain tissue lysate with NPTX2 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry-Paraffin: Neuronal Pentraxin 2 Antibody - BSA Free [NBP1-76899]

Immunohistochemistry-Paraffin: Neuronal Pentraxin 2 Antibody - BSA Free [NBP1-76899]

Immunohistochemistry-Paraffin: Neuronal Pentraxin 2 Antibody [NBP1-76899] - Human brain with NPTX2 antibody at 5 ug/ml.
Neuronal Pentraxin 2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Neuronal Pentraxin 2 Antibody - BSA Free [NBP1-76899] -

Immunocytochemistry/ Immunofluorescence: Neuronal Pentraxin 2 Antibody - BSA Free [NBP1-76899] - Validation of Neuronal Pentraxin 2 in Human Brain Tissue.Immunofluorescent analysis of 4% paraformaldehyde-fixed human brain tissue labeling Neuronal Pentraxin 2 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
Neuronal Pentraxin 2 Antibody - BSA Free

Immunohistochemistry: Neuronal Pentraxin 2 Antibody - BSA Free [NBP1-76899] -

Immunohistochemistry: Neuronal Pentraxin 2 Antibody - BSA Free [NBP1-76899] - Validation of Neuronal Pentraxin 2 in Mouse Brain Tissue.Immunohistochemical analysis of paraffin-embedded mouse brain issue using anti-Neuronal Pentraxin 2 antibody at 5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Neuronal Pentraxin 2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Neuronal Pentraxin 2 Antibody - BSA Free [NBP1-76899] -

Immunocytochemistry/ Immunofluorescence: Neuronal Pentraxin 2 Antibody - BSA Free [NBP1-76899] - Validation of Neuronal Pentraxin 2 in Mouse Brain Tissue.Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain issue labeling Neuronal Pentraxin 2 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).

Applications for Neuronal Pentraxin 2 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Immunohistochemistry

5 ug/mL

Western Blot

0.5-4 ug/mL

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Neuronal Pentraxin 2

Neuronal pentraxin (NPTX) 2 was initially identified as a member of the pentraxin family having high homology to NPTX1. Unlike NPTX1 however, NPTX2 is expressed in testes, pancreas, liver, heart, skeletal muscle as well as brain. NPTX2 possesses the lectin properties common to the pentraxin family, promotes neurite outgrowth, and is rapidly regulated by neuronal activity. NPTX1 and NPTX2 form heterocomplexes that contribute to both activity-independent and -dependent excitatory synaptogenesis. Recently, NPTX2 has been found to be highly upregulated in Parkinsonian substantia nigra and localizes to Lewy bodies and Lewy neurites in sporadic Parkinson's disease (PD), suggesting that it is involved in the pathway dysregulation that underlies PD. This NPTX2 antibody is predicted to be specific to NPTX2 and not recognize NPTX1.

Alternate Names

Apexin, NARP, NPTX2

Gene Symbol

NPTX2

UniProt

NP_002514 (Human)

Additional Neuronal Pentraxin 2 Products

Product Documents for Neuronal Pentraxin 2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Neuronal Pentraxin 2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Acute Phase Proteins

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Protocols

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