Neuronal Pentraxin 2 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-76899
![Western Blot: Neuronal Pentraxin 2 AntibodyBSA Free [NBP1-76899]](https://resources.rndsystems.com/images/products/Neuronal-Pentraxin-2-Antibody-Western-Blot-NBP1-76899-img0011.jpg "Western Blot: Neuronal Pentraxin 2 AntibodyBSA Free [NBP1-76899]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Antibody was raised against a 16 amino acid synthetic peptide near the center of the human NPTX2. The immunogen is located within amino acids 170 - 220 of NPTX2.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Guinea pig (88%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Neuronal Pentraxin 2 Antibody - BSA Free
Western Blot: Neuronal Pentraxin 2 AntibodyBSA Free [NBP1-76899]
Western Blot: Neuronal Pentraxin 2 Antibody [NBP1-76899] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: Neuronal Pentraxin 2 NBP1-76899 (2 ug/mL), Neuronal Pentraxin 2 (4 ug/mL), and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.![Western Blot: Neuronal Pentraxin 2 AntibodyBSA Free [NBP1-76899]](https://resources.rndsystems.com/images/products/Neuronal-Pentraxin-2-Antibody-Western-Blot-NBP1-76899-img0001.jpg "Western Blot: Neuronal Pentraxin 2 AntibodyBSA Free [NBP1-76899]")
Western Blot: Neuronal Pentraxin 2 AntibodyBSA Free [NBP1-76899]
Western Blot: Neuronal Pentraxin 2 Antibody [NBP1-76899] - Mouse brain tissue lysate with NPTX2 antibody at (A) 0.5 and (B) 1 ug/mL.![Western Blot: Neuronal Pentraxin 2 AntibodyBSA Free [NBP1-76899]](https://resources.rndsystems.com/images/products/Neuronal-Pentraxin-2-Antibody-Western-Blot-NBP1-76899-img0006.jpg "Western Blot: Neuronal Pentraxin 2 AntibodyBSA Free [NBP1-76899]")
Western Blot: Neuronal Pentraxin 2 AntibodyBSA Free [NBP1-76899]
Western Blot: Neuronal Pentraxin 2 Antibody [NBP1-76899] - Mouse brain tissue lysate with NPTX2 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.![Immunohistochemistry-Paraffin: Neuronal Pentraxin 2 Antibody - BSA Free [NBP1-76899]](https://resources.rndsystems.com/images/products/Neuronal-Pentraxin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-76899-img0002.jpg "Immunohistochemistry-Paraffin: Neuronal Pentraxin 2 Antibody - BSA Free [NBP1-76899]")
Immunohistochemistry-Paraffin: Neuronal Pentraxin 2 Antibody - BSA Free [NBP1-76899]
Immunohistochemistry-Paraffin: Neuronal Pentraxin 2 Antibody [NBP1-76899] - Human brain with NPTX2 antibody at 5 ug/ml.
Immunocytochemistry/ Immunofluorescence: Neuronal Pentraxin 2 Antibody - BSA Free [NBP1-76899] -
Immunocytochemistry/ Immunofluorescence: Neuronal Pentraxin 2 Antibody - BSA Free [NBP1-76899] - Validation of Neuronal Pentraxin 2 in Human Brain Tissue.Immunofluorescent analysis of 4% paraformaldehyde-fixed human brain tissue labeling Neuronal Pentraxin 2 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
Immunohistochemistry: Neuronal Pentraxin 2 Antibody - BSA Free [NBP1-76899] -
Immunohistochemistry: Neuronal Pentraxin 2 Antibody - BSA Free [NBP1-76899] - Validation of Neuronal Pentraxin 2 in Mouse Brain Tissue.Immunohistochemical analysis of paraffin-embedded mouse brain issue using anti-Neuronal Pentraxin 2 antibody at 5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunocytochemistry/ Immunofluorescence: Neuronal Pentraxin 2 Antibody - BSA Free [NBP1-76899] -
Immunocytochemistry/ Immunofluorescence: Neuronal Pentraxin 2 Antibody - BSA Free [NBP1-76899] - Validation of Neuronal Pentraxin 2 in Mouse Brain Tissue.Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain issue labeling Neuronal Pentraxin 2 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).Applications for Neuronal Pentraxin 2 Antibody - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
20 ug/mL
Immunohistochemistry
5 ug/mL
Western Blot
0.5-4 ug/mL
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Neuronal Pentraxin 2
Additional Neuronal Pentraxin 2 Products
Product Documents for Neuronal Pentraxin 2 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Neuronal Pentraxin 2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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