Neuropilin-1 Antibody (ST05-30)

Novus Biologicals | Catalog # NBP2-67539

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # ST05-30 expressed in HEK293
View all Neuropilin-1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human Neuropilin-1 aa 881-923 / 923. (SwissProt: O14786 Human; SwissProt: P97333 Mouse; SwissProt: Q9QWJ9 Rat)

Localization

Cell membrane, Secreted.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Neuropilin-1 Antibody (ST05-30)

Western Blot: Neuropilin-1 Antibody (ST05-30) [NBP2-67539]

Western Blot: Neuropilin-1 Antibody (ST05-30) [NBP2-67539]

Western Blot: Neuropilin-1 Antibody (ST05-30) [NBP2-67539] - Western blot analysis of Neuropilin-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat A
Immunocytochemistry/ Immunofluorescence: Neuropilin-1 Antibody (ST05-30) [NBP2-67539]

Immunocytochemistry/ Immunofluorescence: Neuropilin-1 Antibody (ST05-30) [NBP2-67539]

Immunocytochemistry/Immunofluorescence: Neuropilin-1 Antibody (ST05-30) [NBP2-67539] - Staining Neuropilin-1 in MCF-7 cells (green) using Neuropilin-1 antibody (ST05-30) [NBP2-67539]. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Neuropilin-1 Antibody (ST05-30) [NBP2-67539]

Immunohistochemistry-Paraffin: Neuropilin-1 Antibody (ST05-30) [NBP2-67539]

Immunohistochemistry-Paraffin: Neuropilin-1 Antibody (ST05-30) [NBP2-67539] - Analysis of paraffin-embedded mouse kidney tissue using Neuropilin-1 antibody (ST05-30) [NBP2-67539]. Counter stained with hematoxylin.
Flow Cytometry: Neuropilin-1 Antibody (ST05-30) [NBP2-67539]

Flow Cytometry: Neuropilin-1 Antibody (ST05-30) [NBP2-67539]

Flow Cytometry: Neuropilin-1 Antibody (ST05-30) [NBP2-67539] - Analysis of Hela cells with Neuropilin-1 antibody (ST05-30) [NBP2-67539] at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence: Neuropilin-1 Antibody (ST05-30) [NBP2-67539]

Immunocytochemistry/ Immunofluorescence: Neuropilin-1 Antibody (ST05-30) [NBP2-67539]

Immunocytochemistry/Immunofluorescence: Neuropilin-1 Antibody (ST05-30) [NBP2-67539] - Staining Neuropilin-1 in HUVEC cells (green) using Neuropilin-1 antibody (ST05-30) [NBP2-67539]. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/ Immunofluorescence: Neuropilin-1 Antibody (ST05-30) [NBP2-67539]

Immunocytochemistry/ Immunofluorescence: Neuropilin-1 Antibody (ST05-30) [NBP2-67539]

Immunocytochemistry/Immunofluorescence: Neuropilin-1 Antibody (ST05-30) [NBP2-67539] - Staining Neuropilin-1 in SHG-44 cells (green) using Neuropilin-1 antibody (ST05-30) [NBP2-67539]. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Neuropilin-1 Antibody (ST05-30) [NBP2-67539]

Immunohistochemistry-Paraffin: Neuropilin-1 Antibody (ST05-30) [NBP2-67539]

Immunohistochemistry-Paraffin: Neuropilin-1 Antibody (ST05-30) [NBP2-67539] - Analysis of paraffin-embedded human kidney tissue using Neuropilin-1 antibody (ST05-30) [NBP2-67539]. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Neuropilin-1 Antibody (ST05-30) [NBP2-67539]

Immunohistochemistry-Paraffin: Neuropilin-1 Antibody (ST05-30) [NBP2-67539]

Immunohistochemistry-Paraffin: Neuropilin-1 Antibody (ST05-30) [NBP2-67539] - Analysis of paraffin-embedded human liver tissue using Neuropilin-1 antibody (ST05-30) [NBP2-67539]. Counter stained with hematoxylin.

Applications for Neuropilin-1 Antibody (ST05-30)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:1000-1:5000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Neuropilin-1

Neuropilin-1 (also known as Npn-1, NRP1, neuropilin, or CD304/BDCA4) is a 923 amino acid (aa) type I transmembrane glycoprotein (theoretical molecular weight 130-140 kDa) located on human chromosome 10p11.22 that encodes multiple intact and soluble isoforms. The 623 aa extracellular domain (ECD) of this human cell surface receptor includes two CUB (complement-binding) domains, two F5/8 domains with homology to coagulation factors V and VIII, and a MAM (meprin) domain. The ECD of human Neuropilin-1 shares 92-95% aa sequence identity with mouse, rat, bovine, and canine Npn-1. The MAM domains of neuropilins are involved in the formation of homo- and hetero-oligomers in the absence of ligands. Neuropilin-1 expression is found in neuronal, endothelial and tumor cells as well as epithelial cells such as in the respiratory, gastrointestinal, lower urological tracts, thyroid, parathyroid and thymus gland (1,2).
Initially found to play an essential role in axon growth and guidance, NRP1 serves as a multifunctional co-receptor for class III semaphorins and heparin-binding members of the VEGF family, participating in regulation of angiogenesis, neuronal development, cell survival, migration and tumor-invasion. Neuropilin-1 has been implicated in viral infections and T-cell activation and is also described as a marker of regulatory T (Treg) cells (3). In COVID-19 infections, studies have shown NRP1 participates in viral infection via NRP1 neutralizing antibodies and binds the SARS-CoV-2 Spike protein, suggesting NRP1 may contribute to viral transport and viral internalization by endocytosis (4).

References

1. Wild JRL, Staton CA, Chapple K, Corfe BM. (2012) Neuropilins: Expression and Roles in the Epithelium. Int J Exp Pathol. 93(2):81-103. PMID: 22414290

2. Bielenberg DR, Pettaway CA, Takashima S, Klagsbrun M. (2006) Neuropilins in Neoplasms: Expression, Regulation, and Function. Exp Cell Res. 312(5):584-93. PMID: 16445911

3. Tordjman R, Lepelletier Y, Lemarchandel V, Cambot M, Gaulard P, Hermine O, Romeo P-H. (2002). A Neuronal Receptor, neuropilin-1, Is Essential for the Initiation of the Primary Immune Response. Nat Immunol. 3(5):477-82. PMID: 11953749

4. Coronavirus research updates: Test frequency matters more than test sensitivity for stopping outbreaks. 2002 June 26. Nature.com

Alternate Names

BDCA-4, CD304, Neuropilin1, NRP1

Gene Symbol

NRP1

Additional Neuropilin-1 Products

Product Documents for Neuropilin-1 Antibody (ST05-30)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Neuropilin-1 Antibody (ST05-30)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Neuropilin-1 Antibody (ST05-30)

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Neuropilin-1 Antibody (ST05-30)

Showing  1 - 3 of 3 FAQs Showing All

  • Q: Does this product contain BSA/Azide?

    A: Yes; the current Neuropilin-1 products we have that contain BSA and azide are NBP2-67539 and NBP2-24617. The former contains 1% BSA along with TBS (pH 7.4) and 40% glycerol as the buffer, and 0.05% Sodium Azide as a preservative. The latter contains 0.05% BSA along with 0.2 ml PBS as the buffer, and 0.05% sodium azide as a preservative.

  • Q: If this product is used in an application or species as a part of a customer review, will that validate this product in the application/species?

    A: If any of our primary antibodes are used in an untested application or species and it is shown to work through images from customer reviews or through publications, this validates the application/species for this product. Please check out our Innovator's Reward Program if you decide to test an antibody with a species or application that is not currently listed.

  • Q: What research areas can Neuropilin-1 antibodies be used in?

    A: Neuropilin-1 products can be applied in the following research areas: Adaptive Immunity, Angiogenesis, Breast Cancer, Cancer, Cardiovascular Biology, Neuroscience, and Virology Bacteria and Parasites.

  • Q: Does this product contain BSA/Azide?

    A: Yes; the current Neuropilin-1 products we have that contain BSA and azide are NBP2-67539 and NBP2-24617. The former contains 1% BSA along with TBS (pH 7.4) and 40% glycerol as the buffer, and 0.05% Sodium Azide as a preservative. The latter contains 0.05% BSA along with 0.2 ml PBS as the buffer, and 0.05% sodium azide as a preservative.

  • Q: If this product is used in an application or species as a part of a customer review, will that validate this product in the application/species?

    A: If any of our primary antibodes are used in an untested application or species and it is shown to work through images from customer reviews or through publications, this validates the application/species for this product. Please check out our Innovator's Reward Program if you decide to test an antibody with a species or application that is not currently listed.

  • Q: What research areas can Neuropilin-1 antibodies be used in?

    A: Neuropilin-1 products can be applied in the following research areas: Adaptive Immunity, Angiogenesis, Breast Cancer, Cancer, Cardiovascular Biology, Neuroscience, and Virology Bacteria and Parasites.

  • Q: Does this product contain BSA/Azide?

    A: Yes; the current Neuropilin-1 products we have that contain BSA and azide are NBP2-67539 and NBP2-24617. The former contains 1% BSA along with TBS (pH 7.4) and 40% glycerol as the buffer, and 0.05% Sodium Azide as a preservative. The latter contains 0.05% BSA along with 0.2 ml PBS as the buffer, and 0.05% sodium azide as a preservative.

  • Q: If this product is used in an application or species as a part of a customer review, will that validate this product in the application/species?

    A: If any of our primary antibodes are used in an untested application or species and it is shown to work through images from customer reviews or through publications, this validates the application/species for this product. Please check out our Innovator's Reward Program if you decide to test an antibody with a species or application that is not currently listed.

  • Q: What research areas can Neuropilin-1 antibodies be used in?

    A: Neuropilin-1 products can be applied in the following research areas: Adaptive Immunity, Angiogenesis, Breast Cancer, Cancer, Cardiovascular Biology, Neuroscience, and Virology Bacteria and Parasites.

Showing  1 - 3 of 3 FAQs Showing All
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