NF-L Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-54669

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (99%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all NF-L Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a Recombinant Protein corresponding to amino acids: SSFSYEPYYSTSYKRRYVETPRVHISSVRSGYSTARSAYSSYSAPVSSSLSVRRSYSSSSGSLMPSLENLDLSQVAAISNDLKSIRTQEKAQLQDLNDRF

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NF-L Antibody - BSA Free

NF-L Antibody

Immunohistochemistry-Paraffin: NF-L Antibody [NBP2-54669] -

Immunohistochemistry-Paraffin: NF-L Antibody [NBP2-54669] -Analysis in human cerebral cortex and skeletal muscle tissues using NBP2-54669 antibody. Corresponding NEFL RNA-seq data are presented for the same tissues.
Western Blot: NF-L Antibody [NBP2-54669]

Western Blot: NF-L Antibody [NBP2-54669]

Western Blot: NF-L Antibody [NBP2-54669] - Analysis in control (vector only transfected HEK293T lysate) and NEFL over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
NF-L Antibody

Immunohistochemistry-Paraffin: NF-L Antibody [NBP2-54669] -

Immunohistochemistry-Paraffin: NF-L Antibody [NBP2-54669] -Staining of human cerebral cortex shows strong positivity in neuronal processes.
NF-L Antibody

Immunohistochemistry-Paraffin: NF-L Antibody [NBP2-54669] -

Immunohistochemistry-Paraffin: NF-L Antibody [NBP2-54669] -Staining of human cerebellum shows strong positivity in neuronal processes in molecular layer.
NF-L Antibody

Immunohistochemistry-Paraffin: NF-L Antibody [NBP2-54669] -

Immunohistochemistry-Paraffin: NF-L Antibody [NBP2-54669] -Staining of human lymph node shows no positivity in non-germinal center cells as expected.
NF-L Antibody

Immunohistochemistry-Paraffin: NF-L Antibody [NBP2-54669] -

Immunohistochemistry-Paraffin: NF-L Antibody [NBP2-54669] -Staining of human skeletal muscle shows no positivity in myocytes as expected.

Applications for NF-L Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NF-L

Neurofilaments are 10nm intermediate filament proteins located in vertebrate neurons, which regulate axonal diameter. They are composed predominantly of the three major neurofilament proteins: NF-Light, NF-Medium and NF-Heavy. NF-L is the light or low molecular weight polypeptide, and it runs on SDS-PAGE gels at about 68kDa, with some size variation in across species.

Antibodies to NF-L may be used to identify NF-L in neurons and to study neuronal processes in tissue sections and/or culture. NF-L antibodies can also be used to study microfilament accumulations seen in many neurological diseases, such as Lou Geri's disease or Alzheimer's disease.

Long Name

Neurofilament Protein, Light Chain

Alternate Names

CMT2E, NEFL, NF68, NFL

Gene Symbol

NEFL

Additional NF-L Products

Product Documents for NF-L Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for NF-L Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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