NF-M Antibody (1B6B12) - BSA Free

Novus Biologicals | Catalog # NBP2-61659

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1B6B12

Format

BSA Free
View all NF-M Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human NF-M (AA: 779-916) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

102.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NF-M Antibody (1B6B12) - BSA Free

Western Blot: NF-M Antibody (1B6B12)BSA Free [NBP2-61659]

Western Blot: NF-M Antibody (1B6B12)BSA Free [NBP2-61659]

Western Blot: NF-M Antibody (1B6B12) [NBP2-61659] - Analysis using NEFM mAb against human NEFM (AA: 779-916) recombinant protein. (Expected MW is *** kDa)
Immunocytochemistry/ Immunofluorescence: NF-M Antibody (1B6B12) - BSA Free [NBP2-61659]

Immunocytochemistry/ Immunofluorescence: NF-M Antibody (1B6B12) - BSA Free [NBP2-61659]

Immunocytochemistry/Immunofluorescence: NF-M Antibody (1B6B12) [NBP2-61659] - Analysis of Hela cells using NEFM mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.
Flow Cytometry: NF-M Antibody (1B6B12) - BSA Free [NBP2-61659]

Flow Cytometry: NF-M Antibody (1B6B12) - BSA Free [NBP2-61659]

Flow Cytometry: NF-M Antibody (1B6B12) [NBP2-61659] - Analysis of Raji cells using NF-M mouse mAb (green) and negative control (red).
Western Blot: NF-M Antibody (1B6B12)BSA Free [NBP2-61659]

Western Blot: NF-M Antibody (1B6B12)BSA Free [NBP2-61659]

Western Blot: NF-M Antibody (1B6B12) [NBP2-61659] - Analysis using NEFM mAb against HEK293 (1) and NEFM (AA: 779-916)-hIgGFc transfected HEK293 (2) cell lysate.
Flow Cytometry: NF-M Antibody (1B6B12) - BSA Free [NBP2-61659]

Flow Cytometry: NF-M Antibody (1B6B12) - BSA Free [NBP2-61659]

Flow Cytometry: NF-M Antibody (1B6B12) [NBP2-61659] - Analysis of Hela cells using NEFM mouse mAb (green) and negative control (red).
ELISA: NF-M Antibody (1B6B12) - BSA Free [NBP2-61659]

ELISA: NF-M Antibody (1B6B12) - BSA Free [NBP2-61659]

ELISA: NF-M Antibody (1B6B12) [NBP2-61659] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
ELISA: NF-M Antibody (1B6B12) - BSA Free [NBP2-61659]

ELISA: NF-M Antibody (1B6B12) - BSA Free [NBP2-61659]

ELISA: NF-M Antibody (1B6B12) [NBP2-61659] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng).

Applications for NF-M Antibody (1B6B12) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Western Blot

1:500-1:2000

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NF-M

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms. ;

Long Name

Neurofilament Protein, Medium Chain

Alternate Names

NEF3, NEFM, NFM

Gene Symbol

NEFM

Additional NF-M Products

Product Documents for NF-M Antibody (1B6B12) - BSA Free

Certificate of Analysis

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Product Specific Notices for NF-M Antibody (1B6B12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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