NF-M Antibody (CL2705) - BSA Free

Novus Biologicals | Catalog # NBP2-46625

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL2705

Format

BSA Free
View all NF-M Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P07197, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for NF-M Antibody (CL2705) - BSA Free

Western Blot: NF-M Antibody (CL2705) [NBP2-46625]

Western Blot: NF-M Antibody (CL2705) [NBP2-46625]

Western Blot: NF-M Antibody (2705) [NBP2-46625] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue
Immunohistochemistry: NF-M Antibody (CL2705) [NBP2-46625]

Immunohistochemistry: NF-M Antibody (CL2705) [NBP2-46625]

Immunohistochemistry: NF-M Antibody (2705) [NBP2-46625] - Staining of human liver shows absence of immunoreactivity (negative control).
Western Blot: NF-M Antibody (CL2705) [NBP2-46625]

Western Blot: NF-M Antibody (CL2705) [NBP2-46625]

Western Blot: NF-M Antibody (2705) [NBP2-46625] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue
Immunohistochemistry: NF-M Antibody (CL2705) [NBP2-46625]

Immunohistochemistry: NF-M Antibody (CL2705) [NBP2-46625]

Immunohistochemistry: NF-M Antibody (2705) [NBP2-46625] - Staining of rat brainstem shows strong immunoreactivity in mesencephalic trigeminal nucleus neurons.
Immunohistochemistry: NF-M Antibody (CL2705) [NBP2-46625]

Immunohistochemistry: NF-M Antibody (CL2705) [NBP2-46625]

Immunohistochemistry: NF-M Antibody (2705) [NBP2-46625] - Staining of mouse cerebral cortex shows strong immunoreactivity in neurofilaments.
Immunohistochemistry: NF-M Antibody (CL2705) [NBP2-46625]

Immunohistochemistry: NF-M Antibody (CL2705) [NBP2-46625]

Immunohistochemistry: NF-M Antibody (2705) [NBP2-46625] - Staining of human cerebellum shows strong immunoreactivity in Purkinje cells and neuronal processes.
Immunohistochemistry: NF-M Antibody (CL2705) [NBP2-46625]

Immunohistochemistry: NF-M Antibody (CL2705) [NBP2-46625]

Immunohistochemistry: NF-M Antibody (2705) [NBP2-46625] - Staining of rat cerebellum shows strong immunoreactivity in neuronal processes.
Immunohistochemistry: NF-M Antibody (CL2705) [NBP2-46625]

Immunohistochemistry: NF-M Antibody (CL2705) [NBP2-46625]

Immunohistochemistry: NF-M Antibody (2705) [NBP2-46625] - Staining of mouse trigeminal ganglion shows strong positivity in primary sensory neurons.
Immunohistochemistry: NF-M Antibody (CL2705) [NBP2-46625]

Immunohistochemistry: NF-M Antibody (CL2705) [NBP2-46625]

Immunohistochemistry: NF-M Antibody (2705) [NBP2-46625] - Staining of human cerebral cortex shows strong positivity in neural fibers.

Applications for NF-M Antibody (CL2705) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:5000 - 1:10000

Immunohistochemistry-Paraffin

1:5000 - 1:10000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NF-M

Neurofilaments are 10nm intermediate filament proteins located in vertebrate neurons, which regulate axonal diameter. They are composed predominantly of the three major neurofilament proteins: NF-Light, NF-Medium and NF-Heavy. NF-M is the middle or medium neurofilament subunit and runs on SDS-PAGE gels in the range 145-170kDa, with some size variation in across species.

Antibodies to NF-M may be used to identify NF-M in neurons and to study neuronal processes in tissue sections and/or culture. NF-M can also be useful in studies of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease.

Long Name

Neurofilament Protein, Medium Chain

Alternate Names

NEF3, NEFM, NFM

Entrez Gene IDs

4741 (Human)

Gene Symbol

NEFM

UniProt

P07197

Additional NF-M Products

Product Documents for NF-M Antibody (CL2705) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for NF-M Antibody (CL2705) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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