NF-M Antibody (CL2705) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-43822
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # CL2705
Format
Azide and BSA Free
Loading...
Product Specifications
Immunogen
This antibody was generated using a recombinant protein sequence of P07197, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for NF-M Antibody (CL2705) - Azide and BSA Free
![NF-M Antibody (CL2705) - Azide and BSA Free Immunohistochemistry-Paraffin: NF-M Antibody [NBP3-43822]](https://resources.rndsystems.com/images/products/nbp3-43822_mouse-nf-m-mab-cl2705-azide-and-bsa-free-282202512173164.jpg "Immunohistochemistry-Paraffin: NF-M Antibody [NBP3-43822]")
Immunohistochemistry-Paraffin: NF-M Antibody [NBP3-43822]
Staining of human cerebellum shows strong immunoreactivity in Purkinje cells and neuronal processes.![NF-M Antibody (CL2705) - Azide and BSA Free Western Blot: NF-M Antibody [NBP3-43822]](https://resources.rndsystems.com/images/products/nbp3-43822_mouse-nf-m-mab-cl2705-azide-and-bsa-free-282202512173124.jpg "Western Blot: NF-M Antibody [NBP3-43822]")
Western Blot: NF-M Antibody [NBP3-43822]
Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Mouse Cerebral Cortex tissue
![NF-M Antibody (CL2705) - Azide and BSA Free Western Blot: NF-M Antibody [NBP3-43822]](https://resources.rndsystems.com/images/products/nbp3-43822_mouse-nf-m-mab-cl2705-azide-and-bsa-free-28220251273712.jpg "Western Blot: NF-M Antibody [NBP3-43822]")
Western Blot: NF-M Antibody [NBP3-43822]
Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human Cerebral Cortex tissue
![NF-M Antibody (CL2705) - Azide and BSA Free Immunohistochemistry-Paraffin: NF-M Antibody [NBP3-43822]](https://resources.rndsystems.com/images/products/nbp3-43822_mouse-nf-m-mab-cl2705-azide-and-bsa-free-282202512362487.jpg "Immunohistochemistry-Paraffin: NF-M Antibody [NBP3-43822]")
Immunohistochemistry-Paraffin: NF-M Antibody [NBP3-43822]
Staining of human liver shows absence of immunoreactivity (negative control).![NF-M Antibody (CL2705) - Azide and BSA Free Immunohistochemistry-Paraffin: NF-M Antibody [NBP3-43822]](https://resources.rndsystems.com/images/products/nbp3-43822_mouse-nf-m-mab-cl2705-azide-and-bsa-free-282202512394427.jpg "Immunohistochemistry-Paraffin: NF-M Antibody [NBP3-43822]")
Immunohistochemistry-Paraffin: NF-M Antibody [NBP3-43822]
Staining of human cerebral cortex shows strong positivity in neural fibers.![NF-M Antibody (CL2705) - Azide and BSA Free Immunohistochemistry-Paraffin: NF-M Antibody [NBP3-43822]](https://resources.rndsystems.com/images/products/nbp3-43822_mouse-nf-m-mab-cl2705-azide-and-bsa-free-282202513245495.jpg "Immunohistochemistry-Paraffin: NF-M Antibody [NBP3-43822]")
Immunohistochemistry-Paraffin: NF-M Antibody [NBP3-43822]
Staining of mouse trigeminal ganglion shows strong positivity in primary sensory neurons.![NF-M Antibody (CL2705) - Azide and BSA Free Immunohistochemistry-Paraffin: NF-M Antibody [NBP3-43822]](https://resources.rndsystems.com/images/products/nbp3-43822_mouse-nf-m-mab-cl2705-azide-and-bsa-free-282202513381559.jpg "Immunohistochemistry-Paraffin: NF-M Antibody [NBP3-43822]")
Immunohistochemistry-Paraffin: NF-M Antibody [NBP3-43822]
Staining of mouse cerebral cortex shows strong immunoreactivity in neurofilaments.![NF-M Antibody (CL2705) - Azide and BSA Free Immunohistochemistry-Paraffin: NF-M Antibody [NBP3-43822]](https://resources.rndsystems.com/images/products/nbp3-43822_mouse-nf-m-mab-cl2705-azide-and-bsa-free-28220251339147.jpg "Immunohistochemistry-Paraffin: NF-M Antibody [NBP3-43822]")
Immunohistochemistry-Paraffin: NF-M Antibody [NBP3-43822]
Staining of rat brainstem shows strong immunoreactivity in mesencephalic trigeminal nucleus neurons.![NF-M Antibody (CL2705) - Azide and BSA Free Immunohistochemistry-Paraffin: NF-M Antibody [NBP3-43822]](https://resources.rndsystems.com/images/products/nbp3-43822_mouse-nf-m-mab-cl2705-azide-and-bsa-free-282202513332353.jpg "Immunohistochemistry-Paraffin: NF-M Antibody [NBP3-43822]")
Immunohistochemistry-Paraffin: NF-M Antibody [NBP3-43822]
Staining of rat cerebellum shows strong immunoreactivity in neuronal processes.Applications for NF-M Antibody (CL2705) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:5000 - 1:10000
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Protein A purified
Reconstitution
Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.
Formulation
Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Calculators
Background: NF-M
Antibodies to NF-M may be used to identify NF-M in neurons and to study neuronal processes in tissue sections and/or culture. NF-M can also be useful in studies of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease.
Long Name
Neurofilament Protein, Medium Chain
Alternate Names
NEF3, NEFM, NFM
Gene Symbol
NEFM
Additional NF-M Products
Product Documents for NF-M Antibody (CL2705) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for NF-M Antibody (CL2705) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Customer Reviews for NF-M Antibody (CL2705) - Azide and BSA Free
There are currently no reviews for this product. Be the first to review NF-M Antibody (CL2705) - Azide and BSA Free and earn rewards!
Have you used NF-M Antibody (CL2705) - Azide and BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...
Associated Pathways
