NFATC1/NFAT2 Antibody (7A6)

Novus Biologicals | Catalog # NB300-620

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Hamster, Primate, Rabbit

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Gel Super Shift Assays

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence, Chemotaxis, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 7A6
View all NFATC1 Primary Antibodies »

Product Specifications

Immunogen

Bacterially expressed glutathione S-transferase (GST) fusion protein containing NF-ATc1 residues 1-654.

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID: 16129432). Hamster reactivity reported in scientific literature (PMID: 10866678). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

Detects cytosolic nuclear factor of activated T-cells (NFAT 2) from transfected human, mouse, and monkey cells. This does not cross react with NFATp.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for NFATC1/NFAT2 Antibody (7A6)

Immunocytochemistry/ Immunofluorescence: NFATC1/NFAT2 Antibody (7A6) [NB300-620]

Immunocytochemistry/ Immunofluorescence: NFATC1/NFAT2 Antibody (7A6) [NB300-620]

Immunocytochemistry/Immunofluorescence: NFATC1/NFAT2 Antibody (7A6) [NB300-620] - NFATc1 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing NFATc1 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Immunohistochemistry-Paraffin: NFATC1/NFAT2 Antibody (7A6) [NB300-620]

Immunohistochemistry-Paraffin: NFATC1/NFAT2 Antibody (7A6) [NB300-620]

Immunohistochemistry-Paraffin: NFATC1/NFAT2 Antibody (7A6) [NB300-620] - Normal biopsies of deparaffinized Human spleen tissue.
Immunocytochemistry/ Immunofluorescence: NFATC1/NFAT2 Antibody (7A6) [NB300-620]

Immunocytochemistry/ Immunofluorescence: NFATC1/NFAT2 Antibody (7A6) [NB300-620]

Immunocytochemistry/Immunofluorescence: NFATC1/NFAT2 Antibody (7A6) [NB300-620] - NFATc1 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing NFATc1 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Immunocytochemistry/ Immunofluorescence: NFATC1/NFAT2 Antibody (7A6) [NB300-620]

Immunocytochemistry/ Immunofluorescence: NFATC1/NFAT2 Antibody (7A6) [NB300-620]

Immunocytochemistry/Immunofluorescence: NFATC1/NFAT2 Antibody (7A6) [NB300-620] - NFATc1 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing NFATc1 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Immunohistochemistry: NFATC1/NFAT2 Antibody (7A6) [NB300-620]

Immunohistochemistry: NFATC1/NFAT2 Antibody (7A6) [NB300-620]

Immunohistochemistry: NFATC1/NFAT2 Antibody (7A6) [NB300-620] - Normal biopsies of deparaffinized Human tonsil tissue.
Immunohistochemistry-Paraffin: NFATC1/NFAT2 Antibody (7A6) [NB300-620]

Immunohistochemistry-Paraffin: NFATC1/NFAT2 Antibody (7A6) [NB300-620]

Immunohistochemistry-Paraffin: NFATC1/NFAT2 Antibody (7A6) [NB300-620] - Normal biopsies of deparaffinized Human skeletal muscle tissue.

Applications for NFATC1/NFAT2 Antibody (7A6)

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

Gel Super Shift Assays

1:1 - 1:100

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:20 - 1:200

Immunohistochemistry-Paraffin

1:20 - 1:200

Immunoprecipitation

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
WB: Detects an approx. 105 kDa band representing transfected NFAT2 from COS cell lysate.

Reviewed Applications

Read 1 review rated 3 using NB300-620 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites diluted with PBS

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NFATC1

The nuclear factor of activated T-cells (NFAT) transcription complex is required for the expression of a group of proteins that coordinate the immune response. NFAT2 is the cytosolic component of NFAT and is encoded by a separate gene. Expression of NFAT2 activates the interleukin (IL-2) promoter in non-T lymphocytes and is required for IL-2 gene expression.

Long Name

Nuclear Factor of Activated T Cells C1

Alternate Names

NFAT2

Entrez Gene IDs

4772 (Human); 18018 (Mouse); 100361818 (Rat)

Gene Symbol

NFATC1

UniProt

O95644

Additional NFATC1 Products

Product Documents for NFATC1/NFAT2 Antibody (7A6)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for NFATC1/NFAT2 Antibody (7A6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for NFATC1/NFAT2 Antibody (7A6)

Customer Reviews for NFATC1/NFAT2 Antibody (7A6) (1)

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Customer Images

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  • NFATC1/NFAT2 Antibody (7A6)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: mouse beta cell line
    Species: Mouse
    Verified Customer | Posted 11/10/2016
    Western blot analysis of mouse beta cell line (betaTC3). NFAT antibody concentration in 10% Non-fat Milk was 1:1000.
    NFATC1/NFAT2 Antibody (7A6) NB300-620

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Protocols

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FAQs for NFATC1/NFAT2 Antibody (7A6)

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  • Q: You list ChIP as an application for this ab (NB300-620). Do you have any data to support it? Which species?

    A: The collaborator we use to produce this monoclonal antibody has validated its use in ChIP via several publications. We do not list them on our datasheets as the products were not obtained directly through us. I am sorry for any confusion. I can provide the listings for you, please see the following links (Human: PMID 15347678 and PMID 19564342), (Mouse: PMID 12065418, PMID 15229217 and PMID 22490438), (Rat: PMID 18667424 and PMID 22566696). As ChIP is listed as a verified application on our datasheet, we will fully guarantee its function for this antibody.

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