NG2/MCSP Antibody (JM10-13)

Western Blot: NG2/MCSP Antibody (JM10-13) [NBP2-66979]

Western Blot: NG2/MCSP Antibody (JM10-13) [NBP2-66979] - Analysis of NG2 on SiHa cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry-Paraffin: NG2/MCSP Antibody (JM10-13) [NBP2-66979]

Immunohistochemistry-Paraffin: NG2/MCSP Antibody (JM10-13) [NBP2-66979] - Analysis of paraffin-embedded human malignant melanoma tissue using anti-NG2/MCSP antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow Cytometry: NG2/MCSP Antibody (JM10-13) [NBP2-66979]

Flow Cytometry: NG2/MCSP Antibody (JM10-13) [NBP2-66979] - Analysis of SHG-44 cells with NG2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.