![Western Blot: NG2/MCSP Antibody [NBP1-21361]](https://resources.rndsystems.com/images/products/NG2-MCSP-Antibody-Western-Blot-NBP1-21361-img0004.jpg "Western Blot: NG2/MCSP Antibody [NBP1-21361]")
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Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Human, Porcine
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 575 and 625 of human melanoma-associated chondroitin sulfate proteoglycan (chondroitin sulfate proteoglycan 4 ) using the numbering given in entry NP_001888.2 (GeneID 1464).
Reactivity Notes
Porcine reactivity reported from a verified customer review.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
The porcine reactivity was reported by an anonymous customer.
Scientific Data Images for NG2/MCSP Antibody
![Immunocytochemistry/ Immunofluorescence: NG2/MCSP Antibody [NBP1-21361]](https://resources.rndsystems.com/images/products/NG2-MCSP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-21361-img0005.jpg "Immunocytochemistry/ Immunofluorescence: NG2/MCSP Antibody [NBP1-21361]")
Immunocytochemistry/ Immunofluorescence: NG2/MCSP Antibody [NBP1-21361]
Immunocytochemistry/Immunofluorescence: NG2/MCSP Antibody [NBP1-21361] - NG2 Antibody [NBP1-21361] - Primary cultured trabecular meshwork cells from porcine eye. Left non stretched TM cells; Right 24 hours stretched TM cells. Annonymous Customer Reported Findings.Applications for NG2/MCSP Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Validated from a verified customer review
Immunoprecipitation
5-10 ug/mg lysate
Western Blot
1:2000-1:10000
Reviewed Applications
Read 1 review rated 5 using NBP1-21361 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: NG2/MCSP
Long Name
Chondroitin Sulfate Proteoglycan NG2/Melanoma Associated Chondroitin Sulfate Proteoglycan
Alternate Names
AN2, CSPG4, HMW-MAA, MCSP, MCSPG, MEL-CSPG
Entrez Gene IDs
1464 (Human)
Gene Symbol
CSPG4
UniProt
Additional NG2/MCSP Products
Product Documents for NG2/MCSP Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for NG2/MCSP Antibody
The porcine reactivity was reported by an anonymous customer.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Customer Reviews for NG2/MCSP Antibody (1)
5 out of 5
1 Customer Rating
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Application: ImmunocytochemistrySample Tested: Primary cultured trabecular meshwork cells from pig eyeSpecies: OtherVerified Customer | Posted 10/19/2011
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
