![NGEF [p Tyr87] Antibody](https://resources.rndsystems.com/images/products/nbp3-23339_rabbit-ngef-p-tyr87-pab-199202312523640.jpg "Immunocytochemistry/Immunofluorescence: NGEF [p Tyr87] Antibody [NBP3-23339] -")
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Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse, Rat, Chicken
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
Phospho-NGEF (Tyr-87) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding tyrosine 87 in mouse NGEF. This sequence has significant homology to the conserved region in human and rat NGEF. [UniProt# Q8CHT1]
Modification
p Tyr87
Specificity
The antibody detects an 80 kDa band corresponding to NGEF in Western blots of human A431 treated with pervanadate. This band is removed by alkaline phosphatase treatment.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for NGEF [p Tyr87] Antibody
Immunocytochemistry/Immunofluorescence: NGEF [p Tyr87] Antibody [NBP3-23339] -
Immunocytochemistry/Immunofluorescence: NGEF [p Tyr87] Antibody [NBP3-23339] - Immunocytochemical labeling of phosphorylated Exphexin-1 in pervanadate-treated mouse C2C12. The cells were labeled with rabbit polyclonal NGEF (C-terminal region) and NGEF (Tyr-87) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.![NGEF [p Tyr87] Antibody](https://resources.rndsystems.com/images/products/nbp3-23339_rabbit-ngef-p-tyr87-pab-1992023141018.jpg "Western Blot: NGEF [p Tyr87] Antibody [NBP3-23339] -")
Western Blot: NGEF [p Tyr87] Antibody [NBP3-23339] -
Western Blot: NGEF [p Tyr87] Antibody [NBP3-23339] - Western blot analysis of human A431 cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 min. (lanes 2 & 4). The blot was probed with anti-NGEF (C-terminal region) (lanes 1 & 2) and anti-NGEF (Tyr-87) (lanes 3 & 4).Applications for NGEF [p Tyr87] Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:200
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: NGEF
Alternate Names
EPHEXIN, ephexin-1, Eph-interacting exchange protein, neuronal guanine nucleotide exchange factorARHGEF27
Gene Symbol
NGEF
Additional NGEF Products
Product Documents for NGEF [p Tyr87] Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for NGEF [p Tyr87] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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