NHERF-1 Antibody

Name: NHERF-1 Antibody
Brand: Novus Biologicals
SKU: NB300-536
Price: 169 USD
Availability: InStock

Novus Biologicals | Catalog # NB300-536

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Citations (3)

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

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Product Specifications

Immunogen

Bacterially expressed, His-tagged human EBP50.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 21761111). Canine reactivity reported in scientific literature (PMID: 21444686).

Specificity

Detects EBP50 from mouse tissues, human T84 and Calu3 cells as well as recombinant human protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NHERF-1 Antibody

Western Blot: NHERF-1 Antibody [NB300-536]

Western Blot: NHERF-1 Antibody [NB300-536] - Analysis of recombinant human EBP50.

Immunocytochemistry/ Immunofluorescence: NHERF-1 Antibody [NB300-536]

NHERF-1-Antibody-Immunocytochemistry-Immunofluorescence-NB300-536-img0007.jpg

Immunohistochemistry: NHERF-1 Antibody [NB300-536]

Immunohistochemistry: NHERF-1 Antibody [NB300-536] - Immunohistochemical staining of NERF-1 / EBP50 in mouse airway.

Immunocytochemistry/ Immunofluorescence: NHERF-1 Antibody [NB300-536] -

Phosphatase and NHERF1 assembly at the immunologic synapse. A-C, Laser-scanning microscopy of CD4+CD25+ T cells stimulated on CD3- and ICAM-1–coated glass slides. Time-dependent accumulation of PHLLP, PTEN, and NHERF1 after anti-CD3–mediated TCR activation was assessed by measuring protein accumulation in the planar layer adjacent to the glass slide. Exposure to ICAM-1 without TCR engagement served as a negative control. Dots represent individual cells analyzed. D, PTEN, NHERF1, and PHLLP subcellular localization in relation to the central supramolecular activation complex (indicated by CD3 staining) and peripheral supramolecular activation complex (indicated by ICAM-1 staining) was analyzed by using TIRF microscopy. Differences were analyzed by using the Mann-Whitney U test. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/27477328), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

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Applications for NHERF-1 Antibody

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

2 ug/mL

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Paraffin

10 ug/mL

Immunoprecipitation

1:10 - 1:500

Western Blot

1 ug/mL

Application Notes

This antibody has been successfully used in Western blot, Immunohistochemistry, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects an ~50 kDa protein representing EBP50 from T84 cell extract. Immunohistochemical staining of EBP50 in mouse airway results in staining of the epithelium. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 14625387).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS, 1 mg/mL BSA

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NHERF-1

EBP 50 [ERM (ezrin-radixin-moesin) binding phosphoprotein of 50 kDa] is a PDZ containing protein that is involved in the linkage of integral membrane proteins to the cytoskeleton. EBP50 contains two tandem PDZ domains followed by a carboxy-terminal sequence that binds to members of the ERM family of membrane-cytoskeleton adaptors. The PDZ domains within EBP50 bind to the carboxy termini of such target proteins as beta2 adrenergic receptor, platelet-derived growth factor receptor (PDGFR), and the cystic fibrosis conductance regulator (CFTR). The PDZ domains are also believed to be involved in the oligomerization of EBP 50 to form multiprotein complexes which helps to facilitate the formation of functional signalling complexes.

EBP 50 has been shown to link integral membrane proteins to the cytoskeleton by binding to members of the ERM family of proteins, which bind to F-actin. Through these interactions, EBP 50 is implicated in the localization of interactive groups of proteins into subcellular domains and in the regulation of activity of those interacting proteins. Immunohistochemical studies have shown that EBP 50 is found exclusively at the apical membranes of epithelial cells.

Alternate Names

EBP50NHERF-1, ezrin-radixin-moesin binding phosphoprotein-50, Ezrin-radixin-moesin-binding phosphoprotein 50, Na(+)/H(+) exchange regulatory cofactor NHE-RF1, Na+/H+ exchange regulatory co-factor, NHERF1solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 1, NHERFNPHLOP2, Regulatory cofactor of Na(+)/H(+) exchanger, sodium/hydrogen exchanger regulatory factor 1, Sodium-hydrogen exchanger regulatory factor 1, solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatoryfactor 1, solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1, Solute carrier family 9 isoform A3 regulatory factor 1

Entrez Gene IDs

9368 (Human); 26941 (Mouse)

Gene Symbol

NHERF1

UniProt

O14745 (Human)

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Product Specific Notices for NHERF-1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for NHERF-1 Antibody

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