NHERF-2 Antibody

Name: NHERF-2 Antibody
Brand: Novus Biologicals
SKU: NB100-2450
Price: 399 USD
Availability: InStock

Novus Biologicals | Catalog # NB100-2450

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

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Product Specifications

Immunogen

Peptide with sequence C-DGSAWKQDPFQ corresponding to internal region according to NP_001123484.1.

Specificity

Please note that this variant has an extra insertion of 11 residues compared to NP_004776.3. The immunizing peptide represents this insertion.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for NHERF-2 Antibody

Western Blot: NHERF-2 Antibody [NB100-2450]

Western Blot: NHERF-2 Antibody [NB100-2450] - (0.3ug/ml) Analysis of human kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: NHERF-2 Antibody [NB100-2450]

Immunohistochemistry-Paraffin: NHERF-2 Antibody [NB100-2450] - IHC analysis of NHERF-2 in paraffin embedded Human Kidney using NB100-2450 at 3ug/ml. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

Applications for NHERF-2 Antibody

Application

Recommended Usage

Immunohistochemistry

1 - 2 ug/ml

Immunohistochemistry-Paraffin

1 - 2 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

0.3 - 1 ug/ml

Application Notes

WB: Approx. 38 kDa band observed in human kidney lysates (calculated MW of 37.4 kDa band according to BAA33216.1). IHC-P: Human kidney shows strong membranous staining in glomerulus-.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NHERF-2

Gene ID(s): 9351 (human)

Alternate Names

E3KARP, Na(+)/H(+) exchange regulatory cofactor NHE-RF2, NHE3RF2, NHERF2MGC104639, NHERF-2NHE3 kinase A regulatory protein E3KARP, OCTS2, SIP-1SRY-interacting protein 1, sodium/hydrogen exchanger, Sodium-hydrogen exchanger regulatory factor 2, solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 2, solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatoryfactor 2, solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 2, Solute carrier family 9 isoform A3 regulatory factor 2, TKA-1SIP1, Tyrosine kinase activator protein 1

Entrez Gene IDs

9351 (Human)

Gene Symbol

SLC9A3R2

UniProt

BAA33216.1 (Human)

Additional NHERF-2 Products

Product Documents for NHERF-2 Antibody

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Certificate of Analysis

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Product Specific Notices for NHERF-2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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