Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody

Novus Biologicals | Catalog # NBP1-52375

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Porcine, Bovine, Canine, Chicken, Equine, Monkey

Cited:

Human

Predicted:

Hamster (94%), Mouse (94%), Opossum (100%), Rabbit (94%), Rat (94%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Nicotinic Acetylcholine R alpha 7/CHRNA7 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence KRPGEDKVRPACQHKQ, from the internal region of the Nicotinic Acetylcholine R alpha 7/CHRNA7 protein sequence according to NP_000737.1.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (100%), Marmoset (88%), Xenopus (88%), Zebrafish (88%), Bat (81%).

Specificity

Human CHRNA7. This antibody also reacts with CHRNA7-FAM7A fusion isoform 1 (NP_647536.1) and isoform 2 (NP_683709.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody

Immunohistochemistry-Paraffin: Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody [NBP1-52375]

Immunohistochemistry-Paraffin: Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody [NBP1-52375]

Immunohistochemistry-Paraffin: Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody [NBP1-52375] - Analysis of anti-CHRNA7 antibody with human hippocampus and isocortex. Image from verified customer review.
Immunohistochemistry-Paraffin: Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody [NBP1-52375]

Immunohistochemistry-Paraffin: Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody [NBP1-52375]

Immunohistochemistry-Paraffin: Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody [NBP1-52375] - Analysis of anti-CHRNA7 antibody with human brain, cortex at concentration 3 ug/ml.

Applications for Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

3-6 ug/ml

Peptide ELISA

1:128000
Application Notes
Use in WB reported in scientific literature (PMID:33850935) IHC: NBP1-52375 was validated for use in IHC on a panel of 21 IHC-P (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity..

Reviewed Applications

Read 1 review rated 5 using NBP1-52375 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

TBS (pH 7.3) and 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Nicotinic Acetylcholine R alpha 7/CHRNA7

The nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediatefast signal transmission at synapses. The nAChRs are thought to be hetero-pentamers composed of homologous subunits.The proposed structure for each subunit is a conserved N-terminal extracellular domain followed by three conservedtransmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminalextracellular region. The protein encoded by this gene forms a homo-oligomeric channel, displays marked permeabilityto calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to,alpha-bungarotoxin. Once this receptor binds acetylcholine, it undergoes an extensive change in conformation thataffects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. This gene islocated in a region identified as a major susceptibility locus for juvenile myoclonic epilepsy and a chromosomallocation involved in the genetic transmission of schizophrenia. An evolutionarily recent partial duplication event inthis region results in a hybrid containing sequence from this gene and a novel FAM7A gene. (provided by RefSeq)

Long Name

Nicotinic Acetylcholine Receptor alpha 7

Alternate Names

CHRNA7, CHRNA7-2, Nicotinic Acetylcholine Ra 7

Entrez Gene IDs

1139 (Human)

Gene Symbol

CHRNA7

UniProt

P36544 (Human)

Additional Nicotinic Acetylcholine R alpha 7/CHRNA7 Products

Product Documents for Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody

Customer Reviews for Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody (1)

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Customer Images

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  • Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Human brian tissue
    Species: Human
    Verified Customer | Posted 04/26/2014
    Nicotinic Acetylcholine Receptor alpha 7 Antibody [NBP1-52375]. Neurons in human hippocampus and isocortex
    Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody NBP1-52375

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody

Showing  1 - 3 of 3 FAQs Showing All

  • Q: I am doing research on Neuroscience and I would like to use some antibodies, especially in Western Blots, but I could do other assays: - alpha7 nicotinic cholinergic receptor; - parvalbumin; - calbindin D28K; The preferred reactivity is anti-human and anti-mouse. Do you have these antibodies? Are they in stock? And what about the prizes: How much are they? How much are the shipping costs? Could you make me any offer?

    A:

    We currently have three alpha7 nicotinic cholingergic receptor antibodies available for purchase. Please see this list for our Parvalbumin antibodies. None are currently validated in mouse. I would like to introduce you to our Innovators Reward Program. We have an excelent calbinin D28K antibody that has been validated for use in human and mouse and WB. The pricing and availability of our products depends on your country.

  • Q: I am looking to buy antibodies against most of the nicotinic receptor subunits for WB and IHC application. Could you please indicate which of your antibodies have been used by other researchers and provide publications showing their use? Thanks.

    A:

    A list of our antibodies directed against he nicotinic acetylcholine receptors can be found here. Any products with publications will be indicated on the search page and can be found under the publications tab on the individual product's datasheet. Furthermore, we guarantee all of our products for the applications and species list on the datasheet. This should help narrow down your search.

  • Q: Would you please tell me whether your antibody Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody (NBP1-52375) is suitable for detecting rat tissue which is PFA perfusion fixed frozen sections?

    A:

    We have not tested this product on frozen sections yet but since it worked well in IHC-P, there is a great chance that it will work on IHC-Fr. Technically speaking, IHC-P protocol poses the target proteins/antigens to more harsh conditions/reagents compared to IHC-Fr, ICC-IF and FLOW protocols. And most of antibodies which work for IHC-P application works for IHC-Fr, ICC-IF and FLOW also. Therefore, I would highly encourage you to test this product in your experiments via our Innovator's Reward program. Innovators Reward

  • Q: I am doing research on Neuroscience and I would like to use some antibodies, especially in Western Blots, but I could do other assays: - alpha7 nicotinic cholinergic receptor; - parvalbumin; - calbindin D28K; The preferred reactivity is anti-human and anti-mouse. Do you have these antibodies? Are they in stock? And what about the prizes: How much are they? How much are the shipping costs? Could you make me any offer?

    A:

    We currently have three alpha7 nicotinic cholingergic receptor antibodies available for purchase. Please see this list for our Parvalbumin antibodies. None are currently validated in mouse. I would like to introduce you to our Innovators Reward Program. We have an excelent calbinin D28K antibody that has been validated for use in human and mouse and WB. The pricing and availability of our products depends on your country.

  • Q: I am looking to buy antibodies against most of the nicotinic receptor subunits for WB and IHC application. Could you please indicate which of your antibodies have been used by other researchers and provide publications showing their use? Thanks.

    A:

    A list of our antibodies directed against he nicotinic acetylcholine receptors can be found here. Any products with publications will be indicated on the search page and can be found under the publications tab on the individual product's datasheet. Furthermore, we guarantee all of our products for the applications and species list on the datasheet. This should help narrow down your search.

  • Q: Would you please tell me whether your antibody Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody (NBP1-52375) is suitable for detecting rat tissue which is PFA perfusion fixed frozen sections?

    A:

    We have not tested this product on frozen sections yet but since it worked well in IHC-P, there is a great chance that it will work on IHC-Fr. Technically speaking, IHC-P protocol poses the target proteins/antigens to more harsh conditions/reagents compared to IHC-Fr, ICC-IF and FLOW protocols. And most of antibodies which work for IHC-P application works for IHC-Fr, ICC-IF and FLOW also. Therefore, I would highly encourage you to test this product in your experiments via our Innovator's Reward program. Innovators Reward

  • Q: I am doing research on Neuroscience and I would like to use some antibodies, especially in Western Blots, but I could do other assays: - alpha7 nicotinic cholinergic receptor; - parvalbumin; - calbindin D28K; The preferred reactivity is anti-human and anti-mouse. Do you have these antibodies? Are they in stock? And what about the prizes: How much are they? How much are the shipping costs? Could you make me any offer?

    A:

    We currently have three alpha7 nicotinic cholingergic receptor antibodies available for purchase. Please see this list for our Parvalbumin antibodies. None are currently validated in mouse. I would like to introduce you to our Innovators Reward Program. We have an excelent calbinin D28K antibody that has been validated for use in human and mouse and WB. The pricing and availability of our products depends on your country.

  • Q: I am looking to buy antibodies against most of the nicotinic receptor subunits for WB and IHC application. Could you please indicate which of your antibodies have been used by other researchers and provide publications showing their use? Thanks.

    A:

    A list of our antibodies directed against he nicotinic acetylcholine receptors can be found here. Any products with publications will be indicated on the search page and can be found under the publications tab on the individual product's datasheet. Furthermore, we guarantee all of our products for the applications and species list on the datasheet. This should help narrow down your search.

  • Q: Would you please tell me whether your antibody Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody (NBP1-52375) is suitable for detecting rat tissue which is PFA perfusion fixed frozen sections?

    A:

    We have not tested this product on frozen sections yet but since it worked well in IHC-P, there is a great chance that it will work on IHC-Fr. Technically speaking, IHC-P protocol poses the target proteins/antigens to more harsh conditions/reagents compared to IHC-Fr, ICC-IF and FLOW protocols. And most of antibodies which work for IHC-P application works for IHC-Fr, ICC-IF and FLOW also. Therefore, I would highly encourage you to test this product in your experiments via our Innovator's Reward program. Innovators Reward

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