Nicotinic Acetylcholine Receptor beta 2 Antibody

Novus Biologicals | Catalog # NBP1-28467

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Nicotinic Acetylcholine R beta 2/CHRNB2 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence QPRHHCARQRLR corresponding to internal region according to NP_000739.1.

Epitope

internal region

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Nicotinic Acetylcholine Receptor beta 2 Antibody

Western Blot: Nicotinic Acetylcholine Receptor beta 2 Antibody [NBP1-28467]

Western Blot: Nicotinic Acetylcholine Receptor beta 2 Antibody [NBP1-28467]

Western Blot: Nicotinic Acetylcholine Receptor beta 2 Antibody [NBP1-28467] - Analysis of Nicotinic Acetylcholine Receptor beta 2 in Human Cerebellum lysate (35ug protein in RIPA buffer) using NBP1-28467 at 1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Nicotinic Acetylcholine Receptor beta 2 Antibody [NBP1-28467]

Immunohistochemistry-Paraffin: Nicotinic Acetylcholine Receptor beta 2 Antibody [NBP1-28467]

Immunohistochemistry-Paraffin: Nicotinic Acetylcholine Receptor beta 2 Antibody [NBP1-28467] - (2.5ug/ml) staining of Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Nicotinic Acetylcholine Receptor beta 2 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

2.5 ug/ml

Peptide ELISA

Detection limit 1:64000

Western Blot

1-3 ug/ml
Application Notes
WB: Approx. 48 kDa band observed in human brain (cerebellum, cerebral cortex) lysates (calculated MW of 57.0 kDa band according to NP_000739.1). The observed molecular weight corresponds to earlier findings with different antibodies from other sources.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Nicotinic Acetylcholine R beta 2/CHRNB2

Neuronal acetylcholine receptors are homo- or heteropentameric complexes composed of homologous alpha and beta subunits. They belong to a superfamily of ligand-gated ion channels which allow the flow of sodium and potassium across the plasma membrane in response to ligands such as acetylcholine and nicotine. This gene encodes one of several beta subunits. Mutations in this gene are associated with autosomal dominant nocturnal frontal lobe epilepsy.

Long Name

Nicotinic Acetylcholine Receptor beta 2

Alternate Names

Cholinergic Receptor Nicotinic Beta 2 Subunit, CHRNB2, EFNL3, NAChRB2, Neuronal Nicotinic Acetylcholine R beta 2

Entrez Gene IDs

1141 (Human); 74493 (Mouse)

Gene Symbol

CHRNB2

UniProt

NP_000739.1 (Human)

Additional Nicotinic Acetylcholine R beta 2/CHRNB2 Products

Product Documents for Nicotinic Acetylcholine Receptor beta 2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Nicotinic Acetylcholine Receptor beta 2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Nicotinic Acetylcholine Receptor beta 2 Antibody

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Nicotinic Acetylcholine Receptor beta 2 Antibody

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  • Q: I am looking to buy antibodies against most of the nicotinic receptor subunits for WB and IHC application. Could you please indicate which of your antibodies have been used by other researchers and provide publications showing their use? Thanks.

    A:

    A list of our antibodies directed against he nicotinic acetylcholine receptors can be found here. Any products with publications will be indicated on the search page and can be found under the publications tab on the individual product's datasheet. Furthermore, we guarantee all of our products for the applications and species list on the datasheet. This should help narrow down your search.

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