Niemann-Pick C1 Antibody (1318A) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Niemann-Pick C1 Antibody (1318A) - Azide and BSA Free [NBP2-80879]

Immunocytochemistry/Immunofluorescence: Niemann-Pick C1 Antibody (1318A) - Azide and BSA Free [NBP2-80879] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-Niemann-Pick C1 Antibody (1318A) at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.

Western Blot: Niemann-Pick C1 Antibody (1318A) - Azide and BSA Free [NBP2-80879]

Western Blot: Niemann-Pick C1 Antibody (1318A) - Azide and BSA Free [NBP2-80879] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Niemann-Pick Type C1/NPC1 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 ug/mL of Rabbit Anti-Human/Mouse/Rat Niemann-Pick Type C1/NPC1 Monoclonal Antibody (Catalog # MAB10105) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Niemann-Pick Type C1/NPC1 at approximately 170-250 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions. Image from the standard format of this antibody.