NIF3L1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-38295

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all NIF3L1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: DFLSRNKQLYQKTEILSLEKPLLLHTGMGRLCTLDESVSLATMIDRIKRHLKLSHIRLALGVGRTLESQVKVVALCAGSGSSV

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%), Rat (89%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NIF3L1 Antibody - BSA Free

Western Blot: NIF3L1 Antibody [NBP2-38295]

Western Blot: NIF3L1 Antibody [NBP2-38295]

Western Blot: NIF3L1 Antibody [NBP2-38295] - Analysis in control (vector only transfected HEK293T lysate) and NIF3L1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: NIF3L1 Antibody [NBP2-38295]

Immunocytochemistry/ Immunofluorescence: NIF3L1 Antibody [NBP2-38295]

Immunocytochemistry/Immunofluorescence: NIF3L1 Antibody [NBP2-38295] - Immunofluorescent staining of human cell line A-431 shows localization to vesicles.
Immunohistochemistry-Paraffin: NIF3L1 Antibody [NBP2-38295]

Immunohistochemistry-Paraffin: NIF3L1 Antibody [NBP2-38295]

Immunohistochemistry-Paraffin: NIF3L1 Antibody [NBP2-38295] - Staining of human tonsil shows strong cytoplasmic and nuclear positivity.

Applications for NIF3L1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NIF3L1

Alternate Names

ALS2CR1, candidate 1, MDS015, NIF3 NGG1 interacting factor 3-like 1 (S. pombe), NIF3-like protein 1, S.pombe homolog)-like 1

Gene Symbol

NIF3L1

UniProt

Q9GZT8 (Human)

Additional NIF3L1 Products

Product Documents for NIF3L1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for NIF3L1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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