NIRF Antibody (3A11) - Azide and BSA Free

Novus Biologicals | Catalog # H00115426-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 3A11

Format

Azide and BSA Free
View all NIRF Primary Antibodies »

Product Specifications

Immunogen

UHRF2 (NP_690856, 81 a.a. ~ 180 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PGTSTQIEAKPCSNSPPKVKKAPRVGPSNQPSTSARARLIDPGFGIYKVNELVDARDVGLGAWFEAHIHSVTRASDGQSRGKTPLKNGSSCKRTNGNIKH

Reactivity Notes

Human. Other species not tested.

Specificity

UHRF2 - ubiquitin-like, containing PHD and RING finger domains, 2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for NIRF Antibody (3A11) - Azide and BSA Free

Western Blot: NIRF Antibody (3A11) [H00115426-M01]

Western Blot: NIRF Antibody (3A11) [H00115426-M01]

Western Blot: NIRF Antibody (3A11) [H00115426-M01] - Analysis of UHRF2 over-expressed 293 cell line, cotransfected with UHRF2 Validated Chimera RNAi ( Cat # H00115426-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with UHRF2 monoclonal antibody (M01), clone 3A11 (Cat # H00115426-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Immunocytochemistry/ Immunofluorescence: NIRF Antibody (3A11) [H00115426-M01]

Immunocytochemistry/ Immunofluorescence: NIRF Antibody (3A11) [H00115426-M01]

Immunocytochemistry/Immunofluorescence: NIRF Antibody (3A11) [H00115426-M01] - Analysis of monoclonal antibody to UHRF2 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: NIRF Antibody (3A11) [H00115426-M01]

Western Blot: NIRF Antibody (3A11) [H00115426-M01]

Western Blot: NIRF Antibody (3A11) [H00115426-M01] - Analysis of UHRF2 expression in transfected 293T cell line by UHRF2 monoclonal antibody (M01), clone 3A11.Lane 1: UHRF2 transfected lysate(56.1 KDa).Lane 2: Non-transfected lysate.

Applications for NIRF Antibody (3A11) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody Reactive against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control. It has also been used for RNAi Validation.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NIRF

This gene encodes a nuclear protein which is involved in cell-cycle regulation. The encoded protein is a ubiquitin-ligase capable of ubiquinating PCNP (PEST-containing nuclear protein), and together they may play a role in tumorigenesis.

Alternate Names

DKFZp434B0920, DKFZp686G0837, E3 ubiquitin-protein ligase UHRF2, EC 6.3.2, EC 6.3.2.-, MGC33463, NIRFUbiquitin-like-containing PHD and RING finger domains protein 2, Np95/ICBP90-like RING finger protein, Np95-like RING finger protein, Nuclear protein 97, Nuclear zinc finger protein Np97, RNF107RING finger protein 107, Ubiquitin-like PHD and RING finger domain-containing protein 2, ubiquitin-like with PHD and ring finger domains 2, ubiquitin-like, containing PHD and RING finger domains, 2, URF2

Entrez Gene IDs

115426 (Human)

Gene Symbol

UHRF2

UniProt

NP_690856 (Human)

Additional NIRF Products

Product Documents for NIRF Antibody (3A11) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for NIRF Antibody (3A11) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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