NKX2.2 Antibody (NX2/1524)

Novus Biologicals | Catalog # NBP2-53207

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # NX2/1524
View all NKX2.2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment (around aa1-119) of human NKX2.2 protein (exact sequence is proprietary) (Uniprot: O95096)

Localization

Nuclear

Marker

Neuroendocrine & Ewing's Sarcoma Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54323)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for NKX2.2 Antibody (NX2/1524)

Immunohistochemistry-Paraffin: NKX2.2 Antibody (NX2/1524) [NBP2-53207]

Immunohistochemistry-Paraffin: NKX2.2 Antibody (NX2/1524) [NBP2-53207]

Immunohistochemistry-Paraffin: NKX2.2 Antibody (NX2/1524) [NBP2-53207] - Formalin-fixed, paraffin-embedded human Ewing's Sarcoma stained with NKX2.2 Monoclonal Antibody (NX2/1524).
Protein Array: NKX2.2 Antibody (NX2/1524) [NBP2-53207]

Protein Array: NKX2.2 Antibody (NX2/1524) [NBP2-53207]

Protein Array: NKX2.2 Antibody (NX2/1524) [NBP2-53207] - Analysis of Protein Array containing >19,000 full-length human proteins using NKX2.2 Antibody (NX2/1524) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.

Applications for NKX2.2 Antibody (NX2/1524)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: NKX2.2

Nkx2.2, or Homeobox protein Nkx-2.2, consists of a 273 amino acid isoform that is 30 kDa, and is involved in controlling and maintaining the expression of genes within pancreatic endocrine cells and axons. Current research is being conducted on the relationship between Nkx2.2 and a variety of diseases and disorders, including glioma, neuronitis, maturity-onset diabetes, pancreatitis, Partington syndrome, sarcoma, gastrointestinal neuroendocrine tumor, acute lymphoblastic leukemia, multiple sclerosis, and carcinoid tumors. The protein has been linked to the Wnt, Hedgehog, and notch pathways, as well as regulation of beta-cell development and gene expression. NKx2.2 interacts with several other proteins, including OLIG2, HDAC1, SIN3A, FOXA2, and FOXO1.

Long Name

NK2 Homeobox 2

Alternate Names

NKX2B

Gene Symbol

NKX2-2

UniProt

O95096 (Human)

Additional NKX2.2 Products

Product Documents for NKX2.2 Antibody (NX2/1524)

Certificate of Analysis

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Product Specific Notices for NKX2.2 Antibody (NX2/1524)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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