NKX2.2 Antibody (NX2/294)

Novus Biologicals | Catalog # NBP2-29432

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Chicken

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # NX2/294
View all NKX2.2 Primary Antibodies »

Product Specifications

Immunogen

Human full-length recombinant NKX2.2 protein (Uniprot: O95096)

Localization

Nuclear

Marker

Neuroendocrine & Ewing's Sarcoma Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-33058)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for NKX2.2 Antibody (NX2/294)

Immunohistochemistry-Paraffin: NKX2.2 Antibody (NX2/294) [NBP2-29432]

Immunohistochemistry-Paraffin: NKX2.2 Antibody (NX2/294) [NBP2-29432]

Immunohistochemistry-Paraffin: NKX2.2 Antibody (NX2/294) [NBP2-29432] - Formalin-fixed, paraffin-embedded Rat Pancreas stained with NKX2.2 Antibody (NX2/294).
Immunohistochemistry-Paraffin: NKX2.2 Antibody (NX2/294) [NBP2-29432]

Immunohistochemistry-Paraffin: NKX2.2 Antibody (NX2/294) [NBP2-29432]

Immunohistochemistry-Paraffin: NKX2.2 Antibody (NX2/294) [NBP2-29432] - Formalin-paraffin pancreas stained with NKX2.2 Ab(NX2/294).
Immunohistochemistry-Frozen: NKX2.2 Antibody (NX2/294) [NBP2-29432]

Immunohistochemistry-Frozen: NKX2.2 Antibody (NX2/294) [NBP2-29432]

Immunohistochemistry-Frozen: NKX2.2 Antibody (NX2/294) [NBP2-29432] - Analysis of NKX2.2 in human pancreas frozen tissue section using NKX2.2 antibody. Image from verified customer review.
Immunohistochemistry-Paraffin: NKX2.2 Antibody (NX2/294) [NBP2-29432]

Immunohistochemistry-Paraffin: NKX2.2 Antibody (NX2/294) [NBP2-29432]

Immunohistochemistry-Paraffin: NKX2.2 Antibody (NX2/294) [NBP2-29432] - Formalin-fixed, paraffin-embedded human Ewing's Sarcoma stained with NKX2.2 Antibody (NX2/294).

Applications for NKX2.2 Antibody (NX2/294)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Frozen

0.5-1.0ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Reviewed Applications

Read 1 review rated 5 using NBP2-29432 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: NKX2.2

The NKX families of homeobox genes are known to act as intermediaries in the neural response to sonic hedgehog signaling during central nervous system development. NKX2.2 is a member of this family of transcription factors and is necessary for neuroendocrine differentiation in the central nervous system and pancreas (Sander M, 2000). This protein was recently identified as a target of EWS-FLI-1, the fusion protein specific to Ewing sarcoma, and was shown to be differentially upregulated in Ewing sarcoma on the basis of array-based gene expression analysis. It also acts as a valuable marker for Ewing sarcoma, with a sensitivity of 93% and a specificity of 89%, and aids in the differential diagnosis of small round cell tumors (Yoshida A, 2012). NKX2.2 preferentially binds the promoter of the alpha cell-specific factor Arx in beta cells when the promoter is in a hypermethylated state. Disruption of the interaction between NKX2.2 and Grgs in mice leads to ectopic expression of Arx in beta cells and is sufficient to cause beta-to-alpha-cell reprogramming during embryonic development and in the adult. These combined in vitro and in vivo studies elucidate how cell-specific DNA modifications and transcriptional regulatory events come together to establish and maintain islet beta cell identity (Papizan JB, 2011).

Long Name

NK2 Homeobox 2

Alternate Names

NKX2B

Gene Symbol

NKX2-2

UniProt

O95096 (Human)

Additional NKX2.2 Products

Product Documents for NKX2.2 Antibody (NX2/294)

Certificate of Analysis

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Product Specific Notices for NKX2.2 Antibody (NX2/294)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for NKX2.2 Antibody (NX2/294) (1)

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  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested:
    Species: Human
    Verified Customer | Posted 09/30/2015
    19 Year old Human pancreas, cryosection.
    NKX2.2 Antibody (NX2/294) NBP2-29432

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Protocols

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FAQs

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