NKX2.2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-82554

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Feline

Cited:

Human, Mouse

Predicted:

Rat (97%). Backed by our 100% Guarantee.

Applications

Validated:

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Knockout Validated, Immunohistochemistry-Paraffin, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all NKX2.2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SLTNTKTGFSVKDILDLPDTNDEEGSVAEGPEEENEGPEPAKRAGPLGQGALDAVQSLPLKNPFYDSSDNPYTRWLASTEGLQYSLHGLAAGAPPQDSSSKSPEPSADESPDNDKETP

Reactivity Notes

Feline, Mouse reactivity reported from verified customer reviews.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NKX2.2 Antibody - BSA Free

Western Blot: NKX2.2 Antibody [NBP1-82554]

Western Blot: NKX2.2 Antibody [NBP1-82554]

Western Blot: NKX2.2 Antibody [NBP1-82554] - Analysis in human cell line U-138 MG.
Immunohistochemistry-Paraffin: NKX2.2 Antibody [NBP1-82554]

Immunohistochemistry-Paraffin: NKX2.2 Antibody [NBP1-82554]

Immunohistochemistry-Paraffin: NKX2.2 Antibody [NBP1-82554] - Staining of human cerebellum shows moderate postivity in cells in molecular layer.
Immunohistochemistry-Paraffin: NKX2.2 Antibody [NBP1-82554]

Immunohistochemistry-Paraffin: NKX2.2 Antibody [NBP1-82554]

Immunohistochemistry-Paraffin: NKX2.2 Antibody [NBP1-82554] - Staining of human pancreas shows strong nuclear positivity in islet cells.
Immunohistochemistry-Paraffin: NKX2.2 Antibody [NBP1-82554]

Immunohistochemistry-Paraffin: NKX2.2 Antibody [NBP1-82554]

Immunohistochemistry-Paraffin: NKX2.2 Antibody [NBP1-82554] - Staining of human cerebral cortex shows strong nuclear postivity in glial cells.
Immunohistochemistry-Paraffin: NKX2.2 Antibody [NBP1-82554]

Immunohistochemistry-Paraffin: NKX2.2 Antibody [NBP1-82554]

Immunohistochemistry-Paraffin: NKX2.2 Antibody [NBP1-82554] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
NKX2.2 Antibody - BSA Free Chromatin Immunoprecipitation ChIP: NKX2.2 Antibody - BSA Free

Chromatin Immunoprecipitation ChIP: NKX2.2 Antibody - BSA Free

ChIP-Exo-Seq composite graph for Anti-NKX2.2 tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh's Lab at Cornell University.

Applications for NKX2.2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Validated from verified customer review.

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Frozen

Validated from verified customer review.

Immunohistochemistry-Paraffin

1:50 - 1:200

Knockout Validated

Validated (bioRxiv Jan 1 2020)

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reviewed Applications

Read 2 reviews rated 4 using NBP1-82554 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NKX2.2

Nkx2.2, or Homeobox protein Nkx-2.2, consists of a 273 amino acid isoform that is 30 kDa, and is involved in controlling and maintaining the expression of genes within pancreatic endocrine cells and axons. Current research is being conducted on the relationship between Nkx2.2 and a variety of diseases and disorders, including glioma, neuronitis, maturity-onset diabetes, pancreatitis, Partington syndrome, sarcoma, gastrointestinal neuroendocrine tumor, acute lymphoblastic leukemia, multiple sclerosis, and carcinoid tumors. The protein has been linked to the Wnt, Hedgehog, and notch pathways, as well as regulation of beta-cell development and gene expression. NKx2.2 interacts with several other proteins, including OLIG2, HDAC1, SIN3A, FOXA2, and FOXO1.

Long Name

NK2 Homeobox 2

Alternate Names

NKX2B

Entrez Gene IDs

4821 (Human)

Gene Symbol

NKX2-2

UniProt

O95096 (Human)

Additional NKX2.2 Products

Product Documents for NKX2.2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for NKX2.2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for NKX2.2 Antibody - BSA Free

Customer Reviews for NKX2.2 Antibody - BSA Free (2)

4 out of 5
2 Customer Ratings
5 Stars
50%
4 Stars
0%
3 Stars
50%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • NKX2.2 Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: Optic nerve
    Species: Feline
    Verified Customer | Posted 06/23/2017
    DAPI(Blue) NBP1-82554(red). Tried 1:50-1:500 concentration. Showed extracelluar, cytoplasmic and nuclear staining.
    4%PFA fixed tissue
    NKX2.2 Antibody - BSA Free NBP1-82554
  • NKX2.2 Antibody
    Name: Jason Spaeth
    Application: Immunohistochemistry-Frozen
    Sample Tested: Mouse pancreas in OCT
    Species: Mouse
    Verified Customer | Posted 11/09/2016
    Staining of Nkx2.2 in mouse pancreas tissue (OCT embed) 1:500 Nkx2.2; 1:1000 Insulin; 1:2000 Glucagon
    NKX2.2 Antibody - BSA Free NBP1-82554

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Protocols

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FAQs

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