NLE1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-83860

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (95%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all NLE1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: TIKVWRAHDGVLCRTLQGHGHWVNTMALSTDYALRTGAFEPAEASVNPQDLQGSLQELKERALSRYNLVRGQGPERLVSGSDDFTL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NLE1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: NLE1 Antibody [NBP1-83860]

Immunocytochemistry/ Immunofluorescence: NLE1 Antibody [NBP1-83860]

Immunocytochemistry/Immunofluorescence: NLE1 Antibody [NBP1-83860] - Staining of human cell line A-431 shows positivity in nucleus and nucleoli.
NLE1 Antibody

Immunohistochemistry-Paraffin: NLE1 Antibody [NBP1-83860] -

Immunohistochemistry-Paraffin: NLE1 Antibody [NBP1-83860] -Staining of human fallopian tube shows moderate nucleoli positivity in glandular cells.
NLE1 Antibody

Immunohistochemistry-Paraffin: NLE1 Antibody [NBP1-83860] -

Immunohistochemistry-Paraffin: NLE1 Antibody [NBP1-83860] -Staining of human cerebral cortex shows moderate nucleoli positivity in neurons.
NLE1 Antibody

Immunohistochemistry-Paraffin: NLE1 Antibody [NBP1-83860] -

Immunohistochemistry-Paraffin: NLE1 Antibody [NBP1-83860] -Staining of human testis shows moderate nucleoli and nuclear positivity in subset of cells in seminiferous ducts.
NLE1 Antibody

Immunohistochemistry-Paraffin: NLE1 Antibody [NBP1-83860] -

Immunohistochemistry-Paraffin: NLE1 Antibody [NBP1-83860] - Staining of human skeletal muscle shows no nuclear positivity in myocytes as expected.
NLE1 Antibody - BSA Free Western Blot: NLE1 Antibody - BSA Free [NBP1-83860]

Western Blot: NLE1 Antibody - BSA Free [NBP1-83860]

Analysis in human cell line A-431.

Applications for NLE1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NLE1

The Notch signaling pathway is an evolutionary conserved system that is involved in intracellular communication. Notch receptors play an important role in development and cell-fate decisions. Notchless is a loss-of-function mutant allele that encodes for protein NLE1 (notchless homolog 1). NLE1 is a 485 amino acid WD40-repeat protein that binds to the cytoplasmic domain of Notch, regulating its signaling activity in Drosophila melanogaster and in mice. Deletion of the NLE1 gene in mice during the early stages of development results in embryonic death, while gene deletion in the late stages of development leads to activation of a caspase-3-dependent apoptotic pathway.In plants, NLE1 is crucial for normal cellular growth and development. Underexpression during shoot proliferation causes pleiotropic defects such as delayed flowering and abnormal organ maturation. It may also play a role in 60S ribosomal subunit biogenesis in yeast. NLE1 contains eight WD40 domains and produces one isoform due to alternative splicing.

Alternate Names

FLJ10458, Nle, Notchless gene homolog, notchless homolog 1 (Drosophila), notchless protein homolog 1

Gene Symbol

NLE1

Additional NLE1 Products

Product Documents for NLE1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for NLE1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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