NLK Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-32264

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Xenopus (98%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all NLK Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human NLK. The exact sequence is proprietary.

Localization

Nucleus, Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NLK Antibody - BSA Free

Western Blot: NLK Antibody [NBP1-32264]

Western Blot: NLK Antibody [NBP1-32264]

Western Blot: NLK Antibody [NBP1-32264] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NLK antibody diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: NLK Antibody [NBP1-32264]

Immunocytochemistry/ Immunofluorescence: NLK Antibody [NBP1-32264]

Immunocytochemistry/Immunofluorescence: NLK Antibody [NBP1-32264] - Paraformaldehyde-fixed HeLa, using antibody at 1:100 dilution.
Immunohistochemistry-Paraffin: NLK Antibody [NBP1-32264]

Immunohistochemistry-Paraffin: NLK Antibody [NBP1-32264]

Immunohistochemistry-Paraffin: NLK Antibody [NBP1-32264] - Paraffin-embedded mouse ovary. NLK antibody [N3C3] ) dilution: 1:500.
Western Blot: NLK Antibody [NBP1-32264]

Western Blot: NLK Antibody [NBP1-32264]

Western Blot: NLK Antibody [NBP1-32264] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:1000.
Western Blot: NLK Antibody [NBP1-32264]

Western Blot: NLK Antibody [NBP1-32264]

Western Blot: NLK Antibody [NBP1-32264] - Sample (30 ug of whole cell lysate) A: Hela 7. 5% SDS PAGE NLK antibody, antibody diluted at 1:1000.

Applications for NLK Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NLK

Role in cell fate determination, required for differentiation of bone marrow stromal cells. Acts downstream of MAP3K7 and HIPK2 to negatively regulate the canonical Wnt/beta-catenin signaling pathway and the phosphorylation and destruction of the MYB transcription factor. May suppress a wide range of transcription factors by phosphorylation of the coactivator, CREBBP. Involved in TGFbeta-mediated mesoderm induction, acting downstream of MAP3K7/TAK1 to phosphorylate STAT3

Alternate Names

DKFZp761G1211, EC 2.7.11, EC 2.7.11.24, FLJ21033, LAK1, nemo like kinase, nemo-like kinaseserine/threonine-protein kinase NLK, Protein LAK1

Gene Symbol

NLK

Additional NLK Products

Product Documents for NLK Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for NLK Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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