NMDAR1 Antibody (R1JHL)

Novus Biologicals | Catalog # NB300-118

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG Clone # R1JHL
View all NMDAR1 Primary Antibodies »

Product Specifications

Immunogen

Fusion protein containing amino acids 1-564 of the NMDAR1 subunit. Accession # P35439

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 20414717). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Marker

Neuronal Marker

Specificity

Specific for endogenous levels of the ~120 kDa NR1 subunit of the NMDA receptor.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Theoretical MW

120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage).

Scientific Data Images for NMDAR1 Antibody (R1JHL)

Western Blot: NMDAR1 Antibody (R1JHL)Azide and BSA Free [NB300-118]

Western Blot: NMDAR1 Antibody (R1JHL)Azide and BSA Free [NB300-118]

Western Blot: NMDAR1 Antibody (R1JHL) [NB300-118] - 10 ug of rat hippocampal lysate showing specific immunolabeling of the ~120 kDa NR1 subunit of the NMDA receptor.
Western Blot: NMDAR1 Antibody (R1JHL)Azide and BSA Free [NB300-118]

Western Blot: NMDAR1 Antibody (R1JHL)Azide and BSA Free [NB300-118]

NMDAR1-Antibody-R1JHL-Western-Blot-NB300-118-img0005.jpg
Immunocytochemistry/ Immunofluorescence: NMDAR1 Antibody (R1JHL) - Azide and BSA Free [NB300-118]

Immunocytochemistry/ Immunofluorescence: NMDAR1 Antibody (R1JHL) - Azide and BSA Free [NB300-118]

Immunocytochemistry/Immunofluorescence: NMDAR1 Antibody (R1JHL) [NB300-118] - NR1 surface staining of mouse corical neruons. This image was submitted via customer Review.
Immunocytochemistry/ Immunofluorescence: NMDAR1 Antibody (R1JHL) - Azide and BSA Free [NB300-118]

Immunocytochemistry/ Immunofluorescence: NMDAR1 Antibody (R1JHL) - Azide and BSA Free [NB300-118]

Immunocytochemistry/Immunofluorescence: NMDAR1 Antibody (R1JHL) [NB300-118] - Mouse neuron stained with anti-NMDAR1 antibody. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: NMDAR1 Antibody (R1JHL) - Azide and BSA Free [NB300-118]

Immunocytochemistry/ Immunofluorescence: NMDAR1 Antibody (R1JHL) - Azide and BSA Free [NB300-118]

Immunocytochemistry/Immunofluorescence: NMDAR1 Antibody (R1JHL) [NB300-118] - Mixed rat cortical cultures (21 DIV) were labelled with anti-NMDAR1 (1:200) and anti-mouse CF568 (1:250). The antibody labels small punctae along the neuronal processes. No distinct features such as spines are visible. This image was submitted via customer Review.
NMDAR1 Antibody (R1JHL) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: NMDAR1 Antibody (R1JHL) - Azide and BSA Free [NB300-118] -

Immunocytochemistry/ Immunofluorescence: NMDAR1 Antibody (R1JHL) - Azide and BSA Free [NB300-118] - Notch1 colocalizes postsynaptically with Reelin signaling components. (A) Representative IEM images from hippocampal slices using an antibody specific for Notch1 show that gold particles are localized at postsynaptic as well as presynaptic membrane terminals. (B) Bar graph summarizing the counting of gold particles on the length of presynaptic & postsynaptic membranes indicates that the majority of the Notch1 gold particles are localized postsynaptically (5*10−3 ± 0.8*10−3 vs. 2*10−3 ± 0.4*10−3, n = 3 mice; Student’s t-test, p < 0.01). (C) Fluorescent immunolabeling on 14 days primary neuronal WT cultures shows colocalization of Notch1 & ApoER2 in soma & processes of pyramidal neurons. Both Notch1 & ApoER2 are similarly co-expressed in NMDAR1 positive puncta (R = 0.92 ± 0.03 & R = 0.86 ± 0.08, respectively; Student’s t-test, p = 0.23). (C′) Close up of a dendrite displaying Notch1, ApoER2 & NMDAR1 expression & “zoom in” captions of puncta showing clustering of the three receptors in teal. (D) Fluorescent immunolabeling on primary neuronal cultures shows that Notch1 & Dab1 localize in the same pyramidal neuron’s soma & processes labeled by phalloidin (R = 0.87 ± 0.06 & R = 0.74 ± 0.07 respectively; Student’s t-test, p = 0.5). (D′) Close up of a dendrite with Notch1, Dab1 & F-actin labeling & “zoom in” captions of dendritic puncta showing clustering of Notch1, Dab1 & F-actin in teal. **p < 0.01. Error bars are SEM & scale bar in (A) is 200 nm for all IEM panels, in (C,D) 50 μm & in (C′,D′) 10 μm & 500 nm in zoom in captions. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26635527), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for NMDAR1 Antibody (R1JHL)

Application
Recommended Usage

Immunohistochemistry-Frozen

1 ug per ml

Western Blot

1:1000
Application Notes
Use in ICC/IF and IHC-F reported in scientific literature (PMID: 20414717). Use in IHC-P reported in scientific literature (PMID 26635527).

Reviewed Applications

Read 14 reviews rated 4.2 using NB300-118 in the following applications:

Formulation, Preparation, and Storage

Purification

Tissue culture supernatant

Reconstitution

Reconstitute with 50 ul PBS to desired concentration.

Formulation

Lyophilized

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NMDAR1

The ion channels activated by glutamate are divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by kainate and a-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR). NMDA receptors are among the most studied receptors in neuroscience because they are involved in neuronal cell development and plasticity, a cellular correlate for learning. NMDA receptors are also implicated in several disorders of the central nervous system including epilepsy and ischemic neuronal cell death. NMDA receptors also appear to be a target for ethanol at physiological concentrations and therefore may play a significant role in alcoholism.

Alternate Names

glutamate [NMDA] receptor subunit zeta-1, glutamate receptor, ionotropic, N-methyl D-aspartate 1, NMDA1, NMDAR1glutamate [NMDA] receptor subunit zeta 1, NMD-R1, N-methyl-D-aspartate receptor channel, subunit zeta-1, N-methyl-D-aspartate receptor subunit NR1, NR1

Entrez Gene IDs

24408 (Rat)

Gene Symbol

GRIN1

UniProt

P35439 (Rat)

Additional NMDAR1 Products

Product Documents for NMDAR1 Antibody (R1JHL)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for NMDAR1 Antibody (R1JHL)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for NMDAR1 Antibody (R1JHL)

Customer Reviews for NMDAR1 Antibody (R1JHL) (14)

4.2 out of 5
14 Customer Ratings
5 Stars
29%
4 Stars
64%
3 Stars
7%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 5 of 14 reviews Showing All
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  • NMDAR1 Antibody (R1JHL)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Mouse brain
    Species: Mouse
    Verified Customer | Posted 01/31/2018
    Brain NR1
    NMDAR1 Antibody (R1JHL) NB300-118
  • NMDAR1 Antibody (R1JHL)
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: Cultured hippocampal neurons
    Species: Mouse
    Verified Customer | Posted 08/04/2017
    NMDAR1 Antibody (R1JHL) NB300-118
  • NMDAR1 Antibody (R1JHL)
    Name: Anonymous
    Application: Immunoprecipitation
    Sample Tested: Mice Brain Lysate
    Species: Mouse
    Verified Customer | Posted 08/04/2017
    NMDAR1 Antibody (R1JHL) NB300-118
  • NMDAR1 Antibody (R1JHL)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: 293T
    Species: Human
    Verified Customer | Posted 08/04/2017
    NMDAR1 Antibody (R1JHL) NB300-118
  • NMDAR1 Antibody (R1JHL)
    Name: Anonymous
    Application: Immunoprecipitation
    Sample Tested: brain and spinal cord
    Species: Mouse
    Verified Customer | Posted 07/14/2017
    NMDAR1 Antibody (R1JHL) NB300-118
  • NMDAR1 Antibody (R1JHL)
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: Cortical neurons
    Species: Mouse
    Verified Customer | Posted 07/14/2017
    NR1 surface staining
    NMDAR1 Antibody (R1JHL) NB300-118
  • NMDAR1 Antibody (R1JHL)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: brain and spinal cord
    Species: Human
    Verified Customer | Posted 07/14/2017
    Human brain NR1
    NMDAR1 Antibody (R1JHL) NB300-118
  • NMDAR1 Antibody (R1JHL)
    Name: Janosch Heller
    Application: Immunocytochemistry
    Sample Tested: rat cortical culture (3 weeks old)
    Species: Rat
    Verified Customer | Posted 06/27/2017
    Mixed rat cortical cultures (21 DIV) were labelled with anti-NMDAR1 (1:200) and anti-mouse CF568 (1:250). The antibody labels small punctae along the neuronal processes. No distinct features such as spines are visible.
    Fixation Solution and Conditions: 4% paraformaldehyde in PBS, 15 minutes RT Blocking Solution & Duration: 3% BSA in PBS with 0.2% saponin, 30 minutes RT Primary Antibody Diluent and Dilutions Tested: 1:200 in 1% BSA in PBS with 0.2% saponin, over night 4°C Secondary Antibody Manufacturer, Host Species, Dilution, & Diluent: Thermo donkey anti-mouse CF568, 1:250 in PBS with 0.2% saponin, 1 hour, RT
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: Mouse neurons
    Species: Mouse
    Verified Customer | Posted 11/26/2015
    NMDAR1 Antibody (R1JHL) NB300-118
  • Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: Mouse neurons
    Species: Mouse
    Verified Customer | Posted 11/26/2015
    NMDAR1 Antibody (R1JHL) NB300-118
  • Name: Anonymous
    Application: Immunoprecipitation
    Sample Tested: Mouse Brain Tissue
    Species: Mouse
    Verified Customer | Posted 10/16/2015
    Co-IP_NR1 and EphB2
    NMDAR1 Antibody (R1JHL) NB300-118
  • Name: Anonymous
    Application: Immunoprecipitation
    Sample Tested: Mouse neuronal cultures
    Species: Mouse
    Verified Customer | Posted 10/15/2015
    Co-IP
    NMDAR1 Antibody (R1JHL) NB300-118
  • Name: Anonymous
    Application: Immunoprecipitation
    Sample Tested: Mouse neurons
    Species: Mouse
    Verified Customer | Posted 10/15/2015
    CoIP
    NMDAR1 Antibody (R1JHL) NB300-118
  • Name: Fugui Li
    Application: Immunofluorescence
    Sample Tested: NR1 plasmid transfected HEK293T cells
    Species: Human
    Verified Customer | Posted 09/30/2014

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