NMDAR2A Antibody (N327/95) - Chimeric - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-52361

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Human, Mouse, Rat, Rabbit

Applications

Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Chicken IgY Clone # N327/95

Format

Azide and BSA Free
View all NMDAR2A Primary Antibodies »

Product Specifications

Immunogen

Fusion protein amino acids 75-325 (extracellular N-terminus) of rat NMDAR2A (Uniprot#: Q00959) produced recombinantly in E. Coli

Specificity

Does not cross-react with GluN2B/NR2B

Clonality

Monoclonal

Host

Chicken

Isotype

IgY

Theoretical MW

170 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N327/95 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N327/95 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in HEK293 cells and then affinity purified from the cell culture media and buffer exchanged into 1X PBS.

Scientific Data Images for NMDAR2A Antibody (N327/95) - Chimeric - Azide and BSA Free

Immunohistochemistry-Paraffin: NMDAR2A Antibody (N327/95) - Chimeric - Azide and BSA Free [NBP3-52361]

Immunohistochemistry-Paraffin: NMDAR2A Antibody (N327/95) - Chimeric - Azide and BSA Free [NBP3-52361]

Representative section of formalin fixed, paraffin-embedded rat brain cerebellum showing cells stained by NBP3-52360 (the BSA Free version of this antibody) (green) using goat anti-chicken IgY secondary antibody. DAPI nuclear stain (blue) shows cell nuclei.
Western Blot: NMDAR2A Antibody (N327/95) - Chimeric - Azide and BSA Free [NBP3-52361]

Western Blot: NMDAR2A Antibody (N327/95) - Chimeric - Azide and BSA Free [NBP3-52361]

Western blotting of rat brain membrane with NBP3-52360 (the BSA Free version of this antibody). The antibody was detected with anti-chicken-HRP and recognizes endogenous NMDAR2A in the rat brain membrane fraction at ~170 kDa.
Immunohistochemistry-Paraffin: NMDAR2A Antibody (N327/95) - Chimeric - Azide and BSA Free [NBP3-52361]

Immunohistochemistry-Paraffin: NMDAR2A Antibody (N327/95) - Chimeric - Azide and BSA Free [NBP3-52361]

Representative section of formalin fixed, paraffin-embedded rat brain hippocampus showing cells stained by NBP3-52360 (the BSA Free version of this antibody) (green) using goat anti-chicken IgY secondary antibody.

Applications for NMDAR2A Antibody (N327/95) - Chimeric - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500-1:1000

Immunohistochemistry-Paraffin

1:500-1:1000

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NMDAR2A

The ion channels activated by glutamate are divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by kainate and a-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR). NMDA receptors are among the most studied receptors in neuroscience because they are involved in neuronal cell development and plasticity, a cellular correlate for learning. NMDA receptors are also implicated in several disorders of the central nervous system including epilepsy and ischemic neuronal cell death. NMDA receptors also appear to be a target for ethanol at physiological concentrations and therefore may play a significant role in alcoholism.

Long Name

Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate, Subunit 2A

Alternate Names

EPND, FESD, GluN2A, glutamate receptor, ionotropic, N-methyl D-aspartate 2A, hNR2A, LKS, NMDA R, NR2A Subunit, NMDA receptor subtype 2A, NMDAR2A, N-methyl D-aspartate receptor subtype 2A, N-methyl-D-aspartate receptor subunit 2A, NR2A, subunit epsilon-1

Gene Symbol

GRIN2A

Additional NMDAR2A Products

Product Documents for NMDAR2A Antibody (N327/95) - Chimeric - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for NMDAR2A Antibody (N327/95) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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