NMDAR2B Antibody

Novus Biologicals | Catalog # NB100-41097

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human, Rat

Predicted:

Canine (100%), Mouse (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Peptide ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Frozen, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all NMDAR2B Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-KDAHEKDDFHHLS corresponding to internal region according to NP_000825.1.

Localization

Cytoskeleton/cytoplasm

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for NMDAR2B Antibody

Western Blot: NMDAR2B Antibody [NB100-41097]

Western Blot: NMDAR2B Antibody [NB100-41097]

Western Blot: NMDAR2B Antibody [NB100-41097] - (2ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: NMDAR2B Antibody [NB100-41097]

Immunocytochemistry/ Immunofluorescence: NMDAR2B Antibody [NB100-41097]

Immunocytochemistry/Immunofluorescence: NMDAR2B Antibody [NB100-41097] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoskeleton and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Applications for NMDAR2B Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Peptide ELISA

Detection limit 1:16000

Western Blot

2 - 4 ug/ml
Application Notes
WB: Approx. 170 kDa band observed in rat brain lysates (calculated MW of 166 kDa band according to NP_000825.1). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 28206989).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NMDAR2B

N-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate receptors. NMDA receptor channel has been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of the key receptor subunit NMDAR1 (GRIN1) and 1 or more of the 4 NMDAR2 subunits: NMDAR2A (GRIN2A), NMDAR2B (GRIN2B), NMDAR2C (GRIN2C), and NMDAR2D (GRIN2D). GRIN2B may be a candidate gene for the neurodegenerative disorder dentato-rubro-pallidoluysian atrophy (DRPLA)

Alternate Names

glutamate [NMDA] receptor subunit epsilon-2, glutamate receptor subunit epsilon-2, glutamate receptor, ionotropic, N-methyl D-aspartate 2B, hNR3, MGC142180, NMDAR2BMGC142178, N-methyl D-aspartate receptor subtype 2B, N-methyl-D-aspartate receptor subunit 3, NR2B, NR3

Entrez Gene IDs

2904 (Human); 14812 (Mouse); 24410 (Rat)

Gene Symbol

GRIN2B

UniProt

NP_000825.1 (Human)

Additional NMDAR2B Products

Product Documents for NMDAR2B Antibody

Certificate of Analysis

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Product Specific Notices for NMDAR2B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for NMDAR2B Antibody

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Protocols

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