NMDAR2B Antibody - C-terminus - Azide Free

Novus Biologicals | Catalog # NB300-106

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide Free
View all NMDAR2B Primary Antibodies »

Product Specifications

Immunogen

Fusion protein from the C-terminal region of the NR2B subunit. Accession # Q00960

Localization

NB300-106 can be used to demonstrate that NR2B is highly enriched in hippocampus compared to either cortex or cerebellum.

Specificity

Specific for the ~180kDa NR2B subunit of the NMDA receptor. Recognizes human, mouse and rat forms of the NR2B subunits of NMDAR. Immunolabeling is blocked by pre-adsorption of antibody with the fusion protein used to generate the antibody. No reactivity towards the NR2A and NR2C subunits.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

180 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

PLEASE NOTE: If 0.01mg size is ordered, product will come lyophilized from 5 mM ammonium bicarbonate. Please reconstitute lyophilized product in 50 ul phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4)

Scientific Data Images for NMDAR2B Antibody - C-terminus - Azide Free

Western Blot: NMDAR2B Antibody [NB300-106]

Western Blot: NMDAR2B Antibody [NB300-106]

NMDAR2B-Antibody-Western-Blot-NB300-106-img0006.jpg
Flow Cytometry: NMDAR2B Antibody [NB300-106]

Flow Cytometry: NMDAR2B Antibody [NB300-106]

Flow Cytometry: NMDAR2B Antibody [NB300-106] - NMDA2B receptor expression on CD4+ T cells. Image supplied by review from confirmed customer.
Western Blot: NMDAR2B Antibody [NB300-106]

Western Blot: NMDAR2B Antibody [NB300-106]

Western Blot: NMDAR2B Antibody [NB300-106]

Applications for NMDAR2B Antibody - C-terminus - Azide Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:1000-1:2000

Immunoprecipitation

3 ul per 200 ug lysate

Western Blot

1:1000
Application Notes
NB300-106 can be used in Western blot where a band is seen at ~ 180 kDa representing the NMDAR2B. Immunohistochemical analysis can be performed using frozen or fixed tissue sections. NB300-106 can also be used to quantitatively immunoprecipitate NMDAR2B where 3 ul will (under appropriate conditions) quantitatively immunoprecipitate all NMDAR2B in 200 mg of rat brain. NB300-106 has also been used successfully in immunofluoresence as reported in the literature (PMID: 20082293) and reported by an anonymous customer review. After reconstitution the antibody should be aliquoted and stored at -20C. Adequate amount of material to conduct 10-mini Western Blots.

Reviewed Applications

Read 3 reviews rated 4 using NB300-106 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Format

Azide Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NMDAR2B

The ion channels activated by glutamate are divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by kainate and a-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR). NMDA receptors are among the most studied receptors in neuroscience because they are involved in neuronal cell development and plasticity, a cellular correlate for learning. NMDA receptors are also implicated in several disorders of the central nervous system including epilepsy and ischemic neuronal cell death. NMDA receptors also appear to be a target for ethanol at physiological concentrations and therefore may play a significant role in alcoholism.

Alternate Names

glutamate [NMDA] receptor subunit epsilon-2, glutamate receptor subunit epsilon-2, glutamate receptor, ionotropic, N-methyl D-aspartate 2B, hNR3, MGC142180, NMDAR2BMGC142178, N-methyl D-aspartate receptor subtype 2B, N-methyl-D-aspartate receptor subunit 3, NR2B, NR3

Entrez Gene IDs

2904 (Human); 14812 (Mouse); 24410 (Rat)

Gene Symbol

GRIN2B

UniProt

Q00960 (Rat)

Additional NMDAR2B Products

Product Documents for NMDAR2B Antibody - C-terminus - Azide Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for NMDAR2B Antibody - C-terminus - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for NMDAR2B Antibody - C-terminus - Azide Free

Customer Reviews for NMDAR2B Antibody - C-terminus - Azide Free (3)

4 out of 5
3 Customer Ratings
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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Name: Anonymous
    Application: Flow Cytometry
    Verified Customer | Posted 01/31/2014
    CD4+ Lymphocytes expressing NMDAR2B
    NMDAR2B Antibody - C-terminus - Azide Free NB300-106
  • Name: Samantha Garcia
    Application: Western Blot
    Sample Tested: Mouse brain synaptosomal membrane
    Species: Mouse
    Verified Customer | Posted 11/06/2013
  • Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: Rat brain tissue
    Species: Other
    Verified Customer | Posted 03/23/2010

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Protocols

View specific protocols for NMDAR2B Antibody - C-terminus - Azide Free (NB300-106):


Western Blot Protocol

Thoroughly sonicate tissue in the following buffer:
1 % SDS (hot, but not boiling, ~ 90C )
10 mM Tris pH 8.0
1 mM EDTA

1. Dilute in appropriate SDS PAGE sample buffer and boil for 5 minutes at 100 C. Let samples cool to room temperature then load onto gel.
2. Run SDS-PAGE per gel apparatus manufacturer?s instructions.
3. Transfer proteins to nitrocellulose or PVDF membrane (if using PVDF, be sure to activate membrane in Methanol prior to use).
4. After transfer, air-dry blot (~15 min.) to more stably fix proteins onto membrane.
5. Block non-specific sites on membrane in 5% NFDM (Non-fat dry milk) -TTBS(Tris-Buffered Saline + 0.1% Tween-20) for 1 hour while shaking at room temperature.
6.Incubate membrane in primary antibody diluted 1:1,000 in 1% NFDM -TTBS while shaking overnight at 4 C.
7. Decant unbound primary antibody solution and wash blot 3 x 10 minutes in TTBS.
8. Incubate blots in appropriate HRP-conjugated (for ECL detection) secondary antibody at a 1:10,000 dilution in 1% NFDM-TTBS for 1 hour while shaking at room temperature.
9. Decant secondary antibody solution and wash blots 3 x 10 minutes in TTBS or use TBS + 0.1% Triton X-100 to reduce excessive background if needed.
10. ECL Detect. (We recommend Pierce?s Super Signal West Dura)

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FAQs for NMDAR2B Antibody - C-terminus - Azide Free

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  • Q: Because I'm looking at staining in a region notorious for high background, they want me to do this control. Could you send me the sequence so that I can have the peptide synthesized?

    A: Unfortunately the sequence for the fusion protein is proprietary.

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