NMT1 Antibody

Novus Biologicals | Catalog # NBP3-12963

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all NMT1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human NMT1. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for NMT1 Antibody

Western Blot: NMT1 Antibody [NBP3-12963]

Western Blot: NMT1 Antibody [NBP3-12963]

Western Blot: NMT1 Antibody [NBP3-12963] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NMT1 antibody (NBP3-12963) diluted at 1:5000.
Immunohistochemistry-Paraffin: NMT1 Antibody [NBP3-12963]

Immunohistochemistry-Paraffin: NMT1 Antibody [NBP3-12963]

Immunohistochemistry-Paraffin: NMT1 Antibody [NBP3-12963] - NMT1 antibody detects NMT1 protein at cytoplasm in rat testis by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. NMT1 antibody (NBP3-12963) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: NMT1 Antibody [NBP3-12963]

Immunohistochemistry-Paraffin: NMT1 Antibody [NBP3-12963]

Immunohistochemistry-Paraffin: NMT1 Antibody [NBP3-12963] - NMT1 antibody detects NMT1 protein at cytoplasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. NMT1 antibody (NBP3-12963) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: NMT1 Antibody [NBP3-12963]

Immunohistochemistry-Paraffin: NMT1 Antibody [NBP3-12963]

Immunohistochemistry-Paraffin: NMT1 Antibody [NBP3-12963] - NMT1 antibody detects NMT1 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. NMT1 antibody (NBP3-12963) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
NMT1 Antibody

Immunocytochemistry/ Immunofluorescence: NMT1 Antibody [NBP3-12963] -

NMT1 antibody detects NMT1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NMT1 protein stained by NMT1 antibody (NBP3-12963) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.

Applications for NMT1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NMT1

Myristate, a rare 14-carbon saturated fatty acid, is cotranslationally attached by an amide linkage to the N-terminal glycine residue of cellular and viral proteins with diverse functions. N-myristoyltransferase (NMT; EC 2.3.1.97) catalyzes the transfer of myristate from CoA to proteins. N-myristoylation appears to be irreversible and is required for full expression of the biologic activities of several N-myristoylated proteins, including the alpha subunit of the signal-transducing guanine nucleotide-binding protein (G protein) GO (GNAO1; MIM 139311) (Duronio et al., 1992 (PubMed 1570339)).(supplied by OMIM)

Alternate Names

alternative, short form NMT-S, glycylpeptide N-tetradecanoyltransferase 1, long form, NMT-L, Myristoyl-CoA:protein N-myristoyltransferase 1, NMT 1, NMTEC 2.3.1.97, N-myristoyltransferase 1, Peptide N-myristoyltransferase 1, Type I N-myristoyltransferase

Gene Symbol

NMT1

Additional NMT1 Products

Product Documents for NMT1 Antibody

Certificate of Analysis

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Product Specific Notices for NMT1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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