Nociceptin Antibody (CL15085) - BSA Free

Novus Biologicals | Catalog # NBP3-43655

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL15085

Format

BSA Free
View all Nociceptin Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of Q13519, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Nociceptin Antibody (CL15085) - BSA Free

Nociceptin Antibody (CL15085) [Unconjugated] Immunohistochemistry-Paraffin: Nociceptin Antibody (CL15085) [NBP3-43655]

Immunohistochemistry-Paraffin: Nociceptin Antibody (CL15085) [NBP3-43655]

Staining of human cerebral cortex shows weak to moderate cytoplasmic positivity in a subset of neurons.
Nociceptin Antibody (CL15085) [Unconjugated] Immunohistochemistry-Paraffin: Nociceptin Antibody (CL15085) [NBP3-43655]

Immunohistochemistry-Paraffin: Nociceptin Antibody (CL15085) [NBP3-43655]

Analysis in human cerebral cortex and placenta tissues using NBP3-43655 antibody. Corresponding PNOC RNA-seq data are presented for the same tissues.
Nociceptin Antibody (CL15085) [Unconjugated] Immunohistochemistry-Paraffin: Nociceptin Antibody (CL15085) [NBP3-43655]

Immunohistochemistry-Paraffin: Nociceptin Antibody (CL15085) [NBP3-43655]

Staining of mouse cerebral cortex shows strong cytoplasmic positivity in neurons.
Nociceptin Antibody (CL15085) [Unconjugated] Immunohistochemistry-Paraffin: Nociceptin Antibody (CL15085) [NBP3-43655]

Immunohistochemistry-Paraffin: Nociceptin Antibody (CL15085) [NBP3-43655]

Staining of human prostate shows no positivity in glandular cells as expected.
Nociceptin Antibody (CL15085) [Unconjugated] Immunohistochemistry-Paraffin: Nociceptin Antibody (CL15085) [NBP3-43655]

Immunohistochemistry-Paraffin: Nociceptin Antibody (CL15085) [NBP3-43655]

Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.
Nociceptin Antibody (CL15085) [Unconjugated] Western blot: Nociceptin Antibody (CL15085) [NBP3-43655]

Western blot: Nociceptin Antibody (CL15085) [NBP3-43655]

Analysis in control (vector only transfected HEK293T lysate) and PNOC over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Nociceptin Antibody (CL15085) [Unconjugated] Immunohistochemistry-Paraffin: Nociceptin Antibody (CL15085) [NBP3-43655]

Immunohistochemistry-Paraffin: Nociceptin Antibody (CL15085) [NBP3-43655]

Staining of human placenta shows no positivity in trophoblastic cells as expected.

Applications for Nociceptin Antibody (CL15085) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, pH 7.2, 40% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nociceptin

Nociceptin (Orphanin FQ, OFQ), a heptadecapeptide peptide, has been designated as an endogenous ligand for the ORL1 receptor. The amino acid sequence of nociceptin hashomology with other opioid peptides, especially the prodynorphin fragment dynorphin A, suggesting a close evolutionary relationship between the precursors. A diffuse distribution throughout the brain is seen with binding studies and in situ hybridization suggesting an extensive role for nociceptin in a multitude of CNS functions. Immunocytochemical localizations in rat spinal cord have demonstrated nociceptin abundance in superficial dorsal horn, lateral spinal nucleus and the region dorsal to the central canal. Nociceptin immunoreactivity was not affected by dorsal rhizotomy, indicating that in spinal cord the peptide is produced by central rather than primary afferent neurons. The localization is supported by its function in processing of nociceptive signals.

Alternate Names

nociceptin, nocistatin, OFQ, P, PPNOC, prepronociceptin, propronociceptin

Gene Symbol

PNOC

Additional Nociceptin Products

Product Documents for Nociceptin Antibody (CL15085) - BSA Free

Certificate of Analysis

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Product Specific Notices for Nociceptin Antibody (CL15085) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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