NOD2 Antibody (2D9) [DyLight 488]

Novus Biologicals | Catalog # NB100-524G

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Primate (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

DyLight 488 (Excitation = 493 nm, Emission = 518 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 2D9
View all NOD2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human NOD2 protein corresponding to residues 28-301. [UniProt# Q9HC29]

Localization

Cytoplasmic

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for NOD2 Antibody (2D9) [DyLight 488]

Immunocytochemistry/Immunofluorescence: NOD2 Antibody (2D9) [DyLight 488] [NB100-524G] - NOD2 was detected in human monocytes using NOD2 antibody (2D9) [DyLight 488]- with a concentration of 1:500 in PBS for 2 hours. Image from verified customer review.
Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [DyLight 488] [NB100-524G] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image for verified customer review.

Applications for NOD2 Antibody (2D9) [DyLight 488]

Application
Recommended Usage

Flow (Intracellular)

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NB100-524G in the following applications:

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: NOD2

NOD2 is a member of the apoptosis regulating protein family that includes caspase recruitment-domains, as well as Apaf-1 and NOD1. It contains two N-terminal CARDs, a nucleotide binding domain (NBD), and multiple C-terminal leucine-rich repeats (LRRs). NOD2 is expressed in monocytes (whereas NOD1 is expressed in multiple tissues). NOD2 plays a role in regulating NF-kappaB, acts as an intracellular receptor for bacterial lipopolysaccharides, and contributes to inflammatory bowel disease (IBD) and Crohn's disease.

Alternate Names

BLAU, CARD15caspase recruitment domain protein 15, caspase recruitment domain family, member 15, Caspase recruitment domain-containing protein 15, CDACUG, CLR16.3, IBD1NLR family, CARD domain containing 2, Inflammatory bowel disease protein 1, NLRC2, NOD-like receptor C2, nucleotide-binding oligomerization domain 2, nucleotide-binding oligomerization domain containing 2, nucleotide-binding oligomerization domain, leucine rich repeat and CARD domaincontaining 2, nucleotide-binding oligomerization domain-containing protein 2, PSORAS1NOD2B

Gene Symbol

NOD2

Additional NOD2 Products

Product Documents for NOD2 Antibody (2D9) [DyLight 488]

Certificate of Analysis

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Product Specific Notices for NOD2 Antibody (2D9) [DyLight 488]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for NOD2 Antibody (2D9) [DyLight 488] (1)

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Customer Images

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  • NOD2 Antibody (2D9) [DyLight 488]
    Name: Christian Ostalecki
    Application: Immunohistochemistry-Frozen
    Sample Tested: Colon tissue (colitis)
    Species: Mouse
    Verified Customer | Posted 05/30/2018
    Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon
    NOD2 Antibody (2D9) [DyLight 488] NB100-524G

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Protocols

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FAQs for NOD2 Antibody (2D9) [DyLight 488]

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  • Q: Can you suggest the best NOD2 antibody for detection of endogenous NOD2 in human fibroblasts?

    A:

    For detecting endogenous NOD2 in human samples, I would recommend NB100-524. This antibody has been validated for use in WB, IHC-Paraffin and IP.

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