Nogo Antibody - BSA Free

Simple Western: Nogo Antibody [NB100-56681]

Simple Western: Nogo Antibody [NB100-56681] - Lane view shows a specific band for Nogo in 0.05 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Western Blot: Nogo Antibody - BSA Free [NB100-56681] -

Generation of cells lacking ERLIN1 and ERLIN2 and identification of the interactome of ERLIN2.(A) Expression levels of ERLIN1 and ERLIN2 mRNAs measured by qRT–PCR normalized for GAPDH in double knock-out (DKO) compared with WT (set to 100). (B) Western blot for ERLIN1 and ERLIN2 in WT, DKO, and DKO+E1/E2. GAPDH was used as a loading control. Asterisks mark unspecific bands. (C) Immunofluorescence staining of ERLIN2 in WT, DKO, and DKO+E1/E2. (D) Western blot for ERLIN1 and ERLIN2 in the input and different fractions of detergent-resistant membrane (DRM)–isolating gradients from WT, DKO, and DKO+E1/E2 cells. FLOT1 and KIDINS220 are DRM markers, RTN4 is a non-DRM protein, and GAPDH is a soluble protein. (E) Volcano plot of endogenous ERLIN2 interactors in WT compared with DKO cells. N = 4 biological replicates. Significantly enriched proteins (Q-value ≤ 0.05) in DKO are labelled in blue. (F) Enriched gene ontology cellular component (GOCC) terms of proteins with Q-value ≤ 0.05 performed using the gProfiler webtool; all proteins identified in the analysis were used as background.Source data are available for this figure. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38782601), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Nogo Antibody - BSA Free [NB100-56681] -

Increased lipid droplet biogenesis buffers lack spastin-M1 at the ER.(A) MEF cell lines were starved in HBSS and treated either with inhibitors of DGAT1 and DGAT2 (D1+D2i) or DMSO overnight and stained with antibodies against reticulon 4. Representative single-plane confocal images. Scale bar: 10 μm. (B) Representative Western blot of spastin levels in the MEF cell lines used. (C) Bar graphs show the percentage of cells possessing collapsed ER in each cell line. Error bars represent SD. One-way ANOVA with post-Tukey test:**P < 0.01, ***P < 0.001. n = 4–5 independent experiments. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32321733), licensed under a CC-BY license. Not internally tested by Novus Biologicals.