NOL12 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-46174
![Western Blot: NOL12 Antibody [NBP1-46174]](https://resources.rndsystems.com/images/products/NOL12-Antibody-Western-Blot-NBP1-46174-img0004.jpg "Western Blot: NOL12 Antibody [NBP1-46174]")
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Loading...
Product Specifications
Immunogen
The immunogen for this product maps to a region between residue 163 and 213 of human Nucleolar Protein 12 using the numbering given in entry NP_077289.1 (GeneID 79159).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for NOL12 Antibody - BSA Free
Western Blot: NOL12 Antibody [NBP1-46174]
Western Blot: NOL12 Antibody [NBP1-46174] - Detection of human and mouse NOL12 by western mlot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NOL12 antibody NBP1-46174 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.![Immunohistochemistry: NOL12 Antibody [NBP1-46174]](https://resources.rndsystems.com/images/products/NOL12-Antibody-Immunohistochemistry-NBP1-46174-img0005.jpg "Immunohistochemistry: NOL12 Antibody [NBP1-46174]")
Immunohistochemistry: NOL12 Antibody [NBP1-46174]
Immunohistochemistry: NOL12 Antibody [NBP1-46174] - Detection of human NOL12 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-NOL12 (NBP1-46174) Detection: DAB![Western Blot: NOL12 Antibody [NBP1-46174]](https://resources.rndsystems.com/images/products/NOL12-Antibody-Western-Blot-NBP1-46174-img0001.jpg "Western Blot: NOL12 Antibody [NBP1-46174]")
Western Blot: NOL12 Antibody [NBP1-46174]
Western Blot: NOL12 Antibody [NBP1-46174] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NOL12 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. NOL12 was also immunoprecipitated by rabbit anti-NOL12 antibody NBP1-46173, which recognizes an upstream epitope.![Immunohistochemistry: NOL12 Antibody [NBP1-46174]](https://resources.rndsystems.com/images/products/NOL12-Antibody-Immunohistochemistry-NBP1-46174-img0003.jpg "Immunohistochemistry: NOL12 Antibody [NBP1-46174]")
Immunohistochemistry: NOL12 Antibody [NBP1-46174]
Immunohistochemistry: NOL12 Antibody [NBP1-46174] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-NOL12 used at a dilution of 1:1,000 (1ug/ml) Detection: DAB staining using Immunohistochemistry Accessory Kit.![Immunoprecipitation: NOL12 Antibody [NBP1-46174]](https://resources.rndsystems.com/images/products/NOL12-Antibody-Immunoprecipitation-NBP1-46174-img0006.jpg "Immunoprecipitation: NOL12 Antibody [NBP1-46174]")
Immunoprecipitation: NOL12 Antibody [NBP1-46174]
Immunoprecipitation: NOL12 Antibody [NBP1-46174] - Detection of human NOL12 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NOL12 antibody A302-733A (lot A302-733A-2) used for IP at 3 ug per reaction. NOL12 was also immunoprecipitated by rabbit anti-NOL12 antibody A302-732A. For blotting immunoprecipitated NOL12, A302-733A was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.Applications for NOL12 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:10-1:500
Immunoprecipitation
2-5 ug/mg lysate
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: NOL12
Alternate Names
dJ37E16.7, FLJ34609, MGC3731, Nop25, nucleolar protein 12, RRP17
Gene Symbol
NOL12
Additional NOL12 Products
Product Documents for NOL12 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for NOL12 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Customer Reviews for NOL12 Antibody - BSA Free
There are currently no reviews for this product. Be the first to review NOL12 Antibody - BSA Free and earn rewards!
Have you used NOL12 Antibody - BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...